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rdf:type |
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lifeskim:mentions |
umls-concept:C0012860,
umls-concept:C0032405,
umls-concept:C0205145,
umls-concept:C0205234,
umls-concept:C0205360,
umls-concept:C0311404,
umls-concept:C0521447,
umls-concept:C1366475,
umls-concept:C1428200,
umls-concept:C1514873,
umls-concept:C1706853,
umls-concept:C1879748
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pubmed:issue |
19
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pubmed:dateCreated |
2003-9-22
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pubmed:abstractText |
The molecular role of poly (ADP-ribose) polymerase-1 in DNA repair is unclear. Here, we show that the single-strand break repair protein XRCC1 is rapidly assembled into discrete nuclear foci after oxidative DNA damage at sites of poly (ADP-ribose) synthesis. Poly (ADP-ribose) synthesis peaks during a 10 min treatment with H2O2 and the appearance of XRCC1 foci peaks shortly afterwards. Both sites of poly (ADP-ribose) and XRCC1 foci decrease to background levels during subsequent incubation in drug-free medium, consistent with the rapidity of the single-strand break repair process. The formation of XRCC1 foci at sites of poly (ADP-ribose) was greatly reduced by mutation of the XRCC1 BRCT I domain that physically interacts with PARP-1. Moreover, we failed to detect XRCC1 foci in Adprt1-/- MEFs after treatment with H2O2. These data demonstrate that PARP-1 is required for the assembly or stability of XRCC1 nuclear foci after oxidative DNA damage and suggest that the formation of these foci is mediated via interaction with poly (ADP-ribose). These results support a model in which the rapid activation of PARP-1 at sites of DNA strand breakage facilitates DNA repair by recruiting the molecular scaffold protein, XRCC1.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-10051636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-10190494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-10191063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-10197627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-10611252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-10667800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-10677679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-10700268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-10767627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-10856830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-10858306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-11016934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-11016956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-11095742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-11163244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-11340626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-11376691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-11440997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-11459959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-11536292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-11707423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-11909950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-11948190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-12509228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-12526788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-12543974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-12606121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-12637553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-1518409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-6889677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-8187089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-8264637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-8532526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-8948628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-8978692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-9001252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-9131015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-9136882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-9207062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-9584196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-9626966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14500814-9786884
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5526-33
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:14500814-Animals,
pubmed-meshheading:14500814-CHO Cells,
pubmed-meshheading:14500814-Cell Nucleus,
pubmed-meshheading:14500814-Cell Survival,
pubmed-meshheading:14500814-Cells, Cultured,
pubmed-meshheading:14500814-Cricetinae,
pubmed-meshheading:14500814-DNA Damage,
pubmed-meshheading:14500814-DNA Repair,
pubmed-meshheading:14500814-DNA-Binding Proteins,
pubmed-meshheading:14500814-Hydrogen Peroxide,
pubmed-meshheading:14500814-Mice,
pubmed-meshheading:14500814-Mutation,
pubmed-meshheading:14500814-Oxidants,
pubmed-meshheading:14500814-Oxidative Stress,
pubmed-meshheading:14500814-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:14500814-Poly Adenosine Diphosphate Ribose,
pubmed-meshheading:14500814-Protein Structure, Tertiary
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pubmed:year |
2003
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pubmed:articleTitle |
A requirement for PARP-1 for the assembly or stability of XRCC1 nuclear foci at sites of oxidative DNA damage.
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