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rdf:type |
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lifeskim:mentions |
umls-concept:C0001175,
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0024204,
umls-concept:C0024400,
umls-concept:C0037224,
umls-concept:C0042769,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C0243192,
umls-concept:C0282554,
umls-concept:C1332822,
umls-concept:C2911684
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pubmed:issue |
4-5
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pubmed:dateCreated |
2003-9-22
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pubmed:abstractText |
Dysregulation of cytokines and chemokines during human immunodeficiency virus 1 (HIV-1) and simian immunodeficiency virus (SIV) infection is thought to be critical in the progression of acquired immunodeficiency syndrome (AIDS). To evaluate the potential role of Th1-agonist chemokines in disease progression during AIDS, we assessed CXCL9/MIG and CXCL10/IP-10 expression simultaneously in the periphery and lymphoid tissues of SIV-infected animals at a single-cell level by flow cytometry. We optimized intracellular staining and analysis of CXCL9/MIG and CXCL10/IP-10 production in human leukocyte antigen (HLA)-DR+ macaque cells by flow cytometry using cross-reactive antibodies against human chemokines. We observed an upregulation of CXCL9/MIG and CXCL10/IP-10 production in both the periphery and lymph nodes of infected animals compared with naïve controls. Animals with higher viral loads had higher levels of CXCL9/MIG and CXCL10/IP-10 producing cells compared with animals with low viral loads. Analysis of cells bearing the receptor (CXCR3) for CXCL9/MIG and CXCL10/IP-10 revealed increased number of CXCR3+ cells in the lymph nodes of infected animals. Importantly, an inverse correlation (P < 0.05) between CXCL9/MIG and CXCL10/IP-10 production, both in the periphery and lymph nodes, and peripheral CD4+ T-cell numbers was observed. These findings provide further evidence that dysregulation of Th1 agonist chemokines might contribute to the ultimate immunopathology during AIDS.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Biotin,
http://linkedlifedata.com/resource/pubmed/chemical/CXCL9 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CXCR3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL10,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL9,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CXCR3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Chemokine,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0047-2565
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
247-64
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:14498985-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:14498985-Animals,
pubmed-meshheading:14498985-Antibodies, Monoclonal,
pubmed-meshheading:14498985-Biotin,
pubmed-meshheading:14498985-Chemokine CXCL10,
pubmed-meshheading:14498985-Chemokine CXCL9,
pubmed-meshheading:14498985-Chemokines,
pubmed-meshheading:14498985-Chemokines, CXC,
pubmed-meshheading:14498985-Flow Cytometry,
pubmed-meshheading:14498985-Gene Expression Regulation,
pubmed-meshheading:14498985-Humans,
pubmed-meshheading:14498985-Immunohistochemistry,
pubmed-meshheading:14498985-In Situ Hybridization,
pubmed-meshheading:14498985-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:14498985-Interferon-gamma,
pubmed-meshheading:14498985-Leukocytes, Mononuclear,
pubmed-meshheading:14498985-Lymph Nodes,
pubmed-meshheading:14498985-Macaca mulatta,
pubmed-meshheading:14498985-Receptors, CXCR3,
pubmed-meshheading:14498985-Receptors, Chemokine,
pubmed-meshheading:14498985-Recombinant Proteins,
pubmed-meshheading:14498985-Simian Acquired Immunodeficiency Syndrome,
pubmed-meshheading:14498985-Viral Load
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pubmed:year |
2003
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pubmed:articleTitle |
Expression of IFN-gamma induced CXCR3 agonist chemokines and compartmentalization of CXCR3+ cells in the periphery and lymph nodes of rhesus macaques during simian immunodeficiency virus infection and acquired immunodeficiency syndrome.
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pubmed:affiliation |
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburg, PA 15261, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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