Linked Life Data

1449211

Source:http://linkedlifedata.com/resource/pubmed/id/1449211

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1992-12-28
pubmed:abstractText
Translation products were used to monitor changes in gene expression between different strains and developmental stages of Aedes aegypti. Total RNA was isolated from fourth stadium larvae, male and female pupae, and male and female adults collected at fixed time intervals following pupation and ecdysis. Differences in RNA populations were assessed by in vitro translation followed by one- and two-dimensional polyacrylamide gel electrophoresis and fluorography. Variations in gene expression between sexes and during development were examined in Liverpool (LVP) and Rockefeller (RKF) Ae. aegypti strains. Sex-related differences consisted primarily of differing lengths of expression for certain polypeptides, although two-dimensional electrophoresis revealed changes in intensity of a 44-kD polypeptide with a pI of 7.9 between males and females. Fourth stadium larvae, pupae, and adults expressed different translation products, which probably correlated with developmental differences. Strain-related differences were observed between LVP and RKF.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0002-9637
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
702-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Variation in Aedes aegypti mRNA populations related to strain, sex, and development.
pubmed:affiliation
Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.