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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1992-12-29
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pubmed:databankReference |
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pubmed:abstractText |
The G-box element is a moderately conserved component of the promoter of many inducible genes, including the alcohol dehydrogenase genes of Arabidopsis and maize. We used monoclonal antibodies generated against partially purified G-box binding factor (GBF) activity to characterize maize proteins that are part of the DNA binding complex. Antibodies interacted with partially purified maize GBF complexes to produce a slower migrating complex in the gel retardation assay. Immunoprecipitation experiments suggested that the protein recognized by the antibody is not a DNA binding protein in and of itself, but rather is associated with the DNA binding complex. These monoclonal antibodies were used to isolate cDNA clones encoding a protein that we have designated GF14. Maize GF14 contains a region resembling a leucine zipper and acidic carboxy and amino termini, of which the latter can form an amphipathic alpha-helix similar to known transcriptional activators such as VP16 and GAL4. Protein gel blot analysis of cell culture extract showed that a single, major protein of approximately 30 kD is recognized by anti-GF14; the protein is also present predominantly in the kernel and root. The deduced amino acid sequence of maize GF14 is more than 80% identical to Arabidopsis GF14 and Oenothera PHP-O, and is more than 60% identical to a class of mammalian brain proteins described as both protein kinase C inhibitors and activators of tyrosine and tryptophan hydroxylases. GF14 is found in a variety of monocotyledons and dicotyledons, gymnosperms, and yeast. This suggests a deep evolutionary conservation of a potential regulatory protein associated with a core sequence found in the promoter region of many genes.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-1498606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-1651913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-16578816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-16661178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-1733782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-1840906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-1846049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-1889090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-1970123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2050116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2102377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2145628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2152112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2152176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2521922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2541432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2562556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2566481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2673023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2683088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2780550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2885229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2902623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2902624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2911757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-2995982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-3028247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-3028647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-3115592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-3135940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-3162770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-3317067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-3678200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-6275366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-642007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-6582470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1446170-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1040-4651
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1295-307
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1446170-14-3-3 Proteins,
pubmed-meshheading:1446170-Amino Acid Sequence,
pubmed-meshheading:1446170-Animals,
pubmed-meshheading:1446170-Base Sequence,
pubmed-meshheading:1446170-Brain Chemistry,
pubmed-meshheading:1446170-Casein Kinases,
pubmed-meshheading:1446170-Cattle,
pubmed-meshheading:1446170-Cloning, Molecular,
pubmed-meshheading:1446170-Conserved Sequence,
pubmed-meshheading:1446170-DNA-Binding Proteins,
pubmed-meshheading:1446170-Gene Expression Regulation,
pubmed-meshheading:1446170-Genes, Plant,
pubmed-meshheading:1446170-Molecular Sequence Data,
pubmed-meshheading:1446170-Nerve Tissue Proteins,
pubmed-meshheading:1446170-Protein Kinases,
pubmed-meshheading:1446170-Sequence Homology, Amino Acid,
pubmed-meshheading:1446170-Species Specificity,
pubmed-meshheading:1446170-Tyrosine 3-Monooxygenase,
pubmed-meshheading:1446170-Zea mays
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pubmed:year |
1992
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pubmed:articleTitle |
A maize protein associated with the G-box binding complex has homology to brain regulatory proteins.
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pubmed:affiliation |
Horticultural Sciences Department, University of Florida, Gainesville 32611.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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