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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1977-11-25
|
| pubmed:abstractText |
The protective effect of ATP, ADP and GTP against the inactivation of Ca2+ + Mg2+ -dependent ATPase by the thiol reagent NBD-chloride is used to calculate the apparent dissociation constants (K'D) of nucleotide enzyme complexes on the basis of a simple kinetic model. The K'D-values of the complexes with Mg-ATP (80 micrometer) and Mg-GTP (500 micrometer) are found to be rather close to their Km-values in the high concentration range supporting maximum activity. The requirement of the occupancy of the low affinity site by Mg ATP for a high rate of the Ca2+ transport system is explained in terms of the flip-flop mechanism established earlier for the analogous Na+ + K+-transporting ATPase system.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0001-5318
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
36
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
K1-10
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:143860-4-Chloro-7-nitrobenzofurazan,
pubmed-meshheading:143860-Adenosine Triphosphatases,
pubmed-meshheading:143860-Animals,
pubmed-meshheading:143860-Binding Sites,
pubmed-meshheading:143860-Calcium,
pubmed-meshheading:143860-Dissociative Disorders,
pubmed-meshheading:143860-Enzyme Repression,
pubmed-meshheading:143860-Humans,
pubmed-meshheading:143860-Magnesium,
pubmed-meshheading:143860-Rabbits,
pubmed-meshheading:143860-Sarcoplasmic Reticulum
|
| pubmed:year |
1977
|
| pubmed:articleTitle |
Determination and functional significance of low affinity nucleotide sites of Ca2+ + Mg2+ -dependent ATPase of sarcoplasmic reticulum.
|
| pubmed:publicationType |
Journal Article
|