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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1992-12-14
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pubmed:abstractText |
Among eukaryotic transcription trans-activators, the human immunodeficiency virus type 1 (HIV-1) Tat protein is exceptional in that its target site TAR is an RNA rather than a DNA sequence. Here, we confirm that fusion of Tat to the RNA-binding domain of the HIV-1 Rev protein permits the efficient activation of an HIV-1 long terminal repeat (LTR) promoter in which critical TAR sequences have been replaced by RNA sequences derived from the HIV-1 Rev response element (RRE). An RRE target sequence as small as 13 nucleotides is shown to form an effective in vivo target for Rev binding. More important, a fusion protein consisting of Rev attached to the VP16 transcription activation domain was also observed to efficiently activate the HIV-1 LTR from this nascent RNA target. These data demonstrate that trans-activation of transcription by acidic activation domains does not require a stable interaction with the promoter DNA and suggest that VP16, like Tat, can act on steps subsequent to the formation of the HIV-1 LTR preinitiation complex. The finding that the activation domains of VP16 and Tat are functionally interchangeable raises the possibility that these apparently disparate viral trans-activators may nevertheless act via similar mechanisms.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, rev,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/rev Gene Products, Human...,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0890-9369
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2077-87
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1427073-Base Sequence,
pubmed-meshheading:1427073-Binding Sites,
pubmed-meshheading:1427073-Cell Line,
pubmed-meshheading:1427073-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:1427073-Gene Products, rev,
pubmed-meshheading:1427073-Gene Products, tat,
pubmed-meshheading:1427073-Genes, rev,
pubmed-meshheading:1427073-Genes, tat,
pubmed-meshheading:1427073-HIV Long Terminal Repeat,
pubmed-meshheading:1427073-HIV-1,
pubmed-meshheading:1427073-HeLa Cells,
pubmed-meshheading:1427073-Humans,
pubmed-meshheading:1427073-Molecular Sequence Data,
pubmed-meshheading:1427073-Nucleic Acid Conformation,
pubmed-meshheading:1427073-Oligodeoxyribonucleotides,
pubmed-meshheading:1427073-Promoter Regions, Genetic,
pubmed-meshheading:1427073-RNA, Viral,
pubmed-meshheading:1427073-Restriction Mapping,
pubmed-meshheading:1427073-Trans-Activators,
pubmed-meshheading:1427073-Transcriptional Activation,
pubmed-meshheading:1427073-rev Gene Products, Human Immunodeficiency Virus,
pubmed-meshheading:1427073-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1992
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pubmed:articleTitle |
The VP16 transcription activation domain is functional when targeted to a promoter-proximal RNA sequence.
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pubmed:affiliation |
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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