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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1992-12-17
|
| pubmed:abstractText |
In recognition of the need for a more comprehensive data base for genetic risk assessment of human exposure to mutagenic agents in the environment, a model system was developed for specific-locus studies in Neurospora crassa. This lower eukaryotic organism permits the utilization of microbial techniques for recovery of large numbers of specific-locus mutations at two closely linked loci as well as their subsequent genetic analysis. In particular, this assay makes possible exploratory experiments with different environmental mutagens to obtain data on a wide variety of experimental conditions. Such data make it possible to study induction kinetics and mutational spectra in a manner that is not as yet feasible in higher eukaryotic organisms. The adenine-3 (ad-3) specific-locus assay was modeled after the 2-gene, morphological specific-locus assay in the dilute-short-ear region of the mouse, and it also detects forward-mutations at two closely linked loci, namely, ad-3A and ad-3B. Because ad-3 mutations are recovered by a direct method, based on the accumulation of a reddish-purple pigment in the vacuoles of the mycelium rather than their requirement for adenine, this system is both a morphological and biochemical specific-locus assay. The use of the ad-3 assay system in experiments with different environmental mutagens has provided precise dose-response curves not only for inactivation, but also the overall induction of ad-3 mutations. Genetic characterization of these ad-3 mutations by a series of 3 rapid and simple genetic tests permits the identification of 18 subclasses of gene/point mutations, and 12 subclasses of multilocus deletion mutations. These subclasses also include 3 different classes of multiple-locus mutations with separate sites of recessive lethal damage either in the immediately adjacent regions or elsewhere in the genome. In summary, this specific-locus assay provides a capability that is unique among eukaryotic organisms for the recovery and analysis of genetic damage at 2 closely linked loci.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0893-6692
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
20
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
225-45
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:1425606-Adenine,
pubmed-meshheading:1425606-Carbon Dioxide,
pubmed-meshheading:1425606-DNA, Fungal,
pubmed-meshheading:1425606-DNA Mutational Analysis,
pubmed-meshheading:1425606-DNA Repair,
pubmed-meshheading:1425606-Dose-Response Relationship, Drug,
pubmed-meshheading:1425606-Environmental Pollutants,
pubmed-meshheading:1425606-Genes, Fungal,
pubmed-meshheading:1425606-Genetic Complementation Test,
pubmed-meshheading:1425606-Genetic Linkage,
pubmed-meshheading:1425606-Genotype,
pubmed-meshheading:1425606-Humans,
pubmed-meshheading:1425606-Models, Genetic,
pubmed-meshheading:1425606-Mutagenesis, Site-Directed,
pubmed-meshheading:1425606-Mutagenicity Tests,
pubmed-meshheading:1425606-Neurospora crassa,
pubmed-meshheading:1425606-Point Mutation,
pubmed-meshheading:1425606-Risk Factors,
pubmed-meshheading:1425606-Sequence Deletion,
pubmed-meshheading:1425606-Stimulation, Chemical
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Development of a specific-locus assay in the ad-3 region of two-component heterokaryons of Neurospora: a review.
|
| pubmed:affiliation |
Center for Life Sciences and Toxicology, Research Triangle Institute, Research Triangle Park, North Carolina 27709.
|
| pubmed:publicationType |
Journal Article,
Review
|