Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-12-18
pubmed:abstractText
Marburg virus was propagated in E6 cells, a cloned cell line of Vero cells, in the presence of [6-3H]glucosamine. Radiolabelled viral glycoprotein was digested with trypsin, and oligosaccharides were liberated by sequential treatment with endo-beta-N-acetylglucosaminidase H, peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F and O-glycosidase, by beta-elimination, and by alkaline hydrolysis. After fractionation by HPLC and gel filtration, glycans were characterized chromatographically, by digestion with exoglycosidases and, in part, by methylation analysis and liquid secondary ion mass spectrometry. The oligosaccharide structures thus established include oligomannosidic and hybrid-type N-glycans, as well as neutral fucosylated bi-, tri- and tetraantennary species, most of which carry an additional bisecting N-acetylglucosamine. In addition, high amounts of neutral mucin-type O-glycans with type-1 and type-2 core structures were detected. None of the glycans present in this viral glycoprotein carried sialic acid residues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Amidohydrolases, http://linkedlifedata.com/resource/pubmed/chemical/Glucosamine, http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Glycoside Hydrolases, http://linkedlifedata.com/resource/pubmed/chemical/Hexosaminidases, http://linkedlifedata.com/resource/pubmed/chemical/Lectins, http://linkedlifedata.com/resource/pubmed/chemical/N-Acetylneuraminic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides, http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments, http://linkedlifedata.com/resource/pubmed/chemical/Peptide-N4-(N-acetyl-beta-glucosamin..., http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides, http://linkedlifedata.com/resource/pubmed/chemical/Sialic Acids, http://linkedlifedata.com/resource/pubmed/chemical/Tritium, http://linkedlifedata.com/resource/pubmed/chemical/Trypsin
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0959-6658
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
299-312
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1421752-Amidohydrolases, pubmed-meshheading:1421752-Animals, pubmed-meshheading:1421752-Carbohydrate Conformation, pubmed-meshheading:1421752-Carbohydrate Sequence, pubmed-meshheading:1421752-Chromatography, Gel, pubmed-meshheading:1421752-Chromatography, High Pressure Liquid, pubmed-meshheading:1421752-Glucosamine, pubmed-meshheading:1421752-Glycoproteins, pubmed-meshheading:1421752-Glycoside Hydrolases, pubmed-meshheading:1421752-Hexosaminidases, pubmed-meshheading:1421752-Hydrogen-Ion Concentration, pubmed-meshheading:1421752-Lectins, pubmed-meshheading:1421752-Marburgvirus, pubmed-meshheading:1421752-Methylation, pubmed-meshheading:1421752-Molecular Sequence Data, pubmed-meshheading:1421752-Molecular Structure, pubmed-meshheading:1421752-N-Acetylneuraminic Acid, pubmed-meshheading:1421752-Oligosaccharides, pubmed-meshheading:1421752-Peptide Fragments, pubmed-meshheading:1421752-Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase, pubmed-meshheading:1421752-Polysaccharides, pubmed-meshheading:1421752-Sialic Acids, pubmed-meshheading:1421752-Tritium, pubmed-meshheading:1421752-Trypsin, pubmed-meshheading:1421752-Vero Cells
pubmed:year
1992
pubmed:articleTitle
Carbohydrate structure of Marburg virus glycoprotein.
pubmed:affiliation
Biochemisches Institut am Klinikum der Universität, Giessen, FRG.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't