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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1992-11-13
|
| pubmed:abstractText |
Male and female Holtzman rats were exposed to ethylbenzene, and the effect on liver microsomal activities was studied. Hydrocarbon- and sex-dependent effects on P450-dependent metabolism of drugs and aromatic hydrocarbons were investigated. Hydrocarbon treatment produced two patterns of induction in cytochrome P450-dependent activities: (1) induction common to both sexes; and (2) induction exclusively in females. Benzphetamine N-demethylation, 7-ethoxycoumarin O-deethylation, p-nitroanisole O-demethylation and aromatic hydroxylation of toluene were induced in both sexes after rats were exposed to ethylbenzene. The rate of benzphetamine N-demethylation increased 4-fold in females and nearly doubled in males. The increase in O-deethylation of 7-ethoxycoumarin was 3-fold in females and doubled in males, while p-nitroanisole O-demethylation increased 4-fold in both sexes after exposure to ethylbenzene. Ethylbenzene had its greatest effect upon the formation of aromatic hydroxylated metabolites of toluene. Ethylbenzene exposure increased the rate of o-cresol formation by 4- and 9-fold in female and male rats, respectively. The formation rate of p-cresol was undetectable in either sex prior to hydrocarbon exposure; however, after the rats were given ethylbenzene, rates increased to 0.4 nmol/min/mg protein in females and to 0.9 nmol/min/mg protein in the males. Ethylbenzene exposure selectively induced aminopyrine demethylation, aniline hydroxylation, N,N-dimethylnitrosamine N-demethylation (DMNA) and aliphatic hydroxylation of toluene in females. Rates for aminopyrine, aniline, and DMNA were increased 50% over controls, while formation of benzyl alcohol from toluene was enhanced to 260% of control. Western immunoblotting indicated that ethylbenzene treatment induced cytochrome P450 2B1/2B2 to a greater extent in male rats and cytochrome P450 2E1 only in females. Ethylbenzene exposure did not affect significantly the level of cytochrome P450 1A1.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-cresol,
http://linkedlifedata.com/resource/pubmed/chemical/4-cresol,
http://linkedlifedata.com/resource/pubmed/chemical/Benzene Derivatives,
http://linkedlifedata.com/resource/pubmed/chemical/Benzyl Alcohol,
http://linkedlifedata.com/resource/pubmed/chemical/Benzyl Alcohols,
http://linkedlifedata.com/resource/pubmed/chemical/Cresols,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 CYP2E1,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes b5,
http://linkedlifedata.com/resource/pubmed/chemical/NADH Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidoreductases, N-Demethylating,
http://linkedlifedata.com/resource/pubmed/chemical/Toluene,
http://linkedlifedata.com/resource/pubmed/chemical/ethylbenzene
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0006-2952
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
44
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1171-82
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1417939-Animals,
pubmed-meshheading:1417939-Benzene Derivatives,
pubmed-meshheading:1417939-Benzyl Alcohol,
pubmed-meshheading:1417939-Benzyl Alcohols,
pubmed-meshheading:1417939-Blotting, Western,
pubmed-meshheading:1417939-Cresols,
pubmed-meshheading:1417939-Cytochrome P-450 CYP2E1,
pubmed-meshheading:1417939-Cytochrome P-450 Enzyme System,
pubmed-meshheading:1417939-Cytochromes b5,
pubmed-meshheading:1417939-Enzyme Induction,
pubmed-meshheading:1417939-Female,
pubmed-meshheading:1417939-Hydroxylation,
pubmed-meshheading:1417939-Male,
pubmed-meshheading:1417939-Microsomes, Liver,
pubmed-meshheading:1417939-NADH Dehydrogenase,
pubmed-meshheading:1417939-Oxidoreductases, N-Demethylating,
pubmed-meshheading:1417939-Rats,
pubmed-meshheading:1417939-Sex Factors,
pubmed-meshheading:1417939-Toluene
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Ethylbenzene-mediated induction of cytochrome P450 isozymes in male and female rats.
|
| pubmed:affiliation |
Department of Pharmacology, Louisiana State University Medical Center, New Orleans 70112.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|