rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
9
|
pubmed:dateCreated |
1992-10-30
|
pubmed:abstractText |
We developed a polymerase chain reaction method in order to detect and identify both Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica. Polymerase chain reaction was performed by using a mixture of primers against the inv gene from Y. pseudotuberculosis and the ail gene from pathogenic Y. enterocolitica. Further addition of primers against the plasmid-coded virF gene from Y. enterocolitica made it possible to detect a virulence-associated gene of both species at the same time. This method was proved to be an adequate and convenient procedure for routine detection and identification of these bacilli.
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pubmed:commentsCorrections |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0095-1137
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
30
|
pubmed:geneSymbol |
ail,
f1p6,
virF
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2484-6
|
pubmed:dateRevised |
2010-9-7
|
pubmed:meshHeading |
pubmed-meshheading:1401022-Animals,
pubmed-meshheading:1401022-Bacterial Typing Techniques,
pubmed-meshheading:1401022-Base Sequence,
pubmed-meshheading:1401022-Deoxyribonucleotides,
pubmed-meshheading:1401022-Dogs,
pubmed-meshheading:1401022-Genes, Bacterial,
pubmed-meshheading:1401022-Humans,
pubmed-meshheading:1401022-Molecular Sequence Data,
pubmed-meshheading:1401022-Oligonucleotides,
pubmed-meshheading:1401022-Polymerase Chain Reaction,
pubmed-meshheading:1401022-Sensitivity and Specificity,
pubmed-meshheading:1401022-Virulence,
pubmed-meshheading:1401022-Yersinia,
pubmed-meshheading:1401022-Yersinia enterocolitica,
pubmed-meshheading:1401022-Yersinia pseudotuberculosis
|
pubmed:year |
1992
|
pubmed:articleTitle |
Detection and identification of Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica by an improved polymerase chain reaction method.
|
pubmed:affiliation |
Department of Microbiology, Okayama Prefectural Institute for Environmental Science and Public Health, Japan.
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pubmed:publicationType |
Journal Article
|