Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-11-13
pubmed:abstractText
Northern-blot analyses demonstrated a strong gene expression of ATp citrate-lyase in liver and adipose tissue of rat and a weak expression in brain, heart, small intestine and muscle. After refeeding a carbohydrate/protein diet to fasted rats, the transcriptional rate had already increased within 2 h, the mRNA concentration reached a maximal level of approximately 30-fold increased in 16 h, and the enzyme induction increased sixfold in 48 h. By feeding only carbohydrate without protein, the transcriptional rate was increased threefold, and the mRNA concentration and enzyme induction comparably, to the levels in the carbohydrate/protein diet. It appears that protein feeding is not necessary to induce ATP citrate-lyase. In diabetic rats fed on a glucose diet, the transcriptional rate, mRNA concentration and enzyme level were very low in comparison with the normal. By fructose feeding, however, the transcriptional rate was more greatly increased and the mRNA concentration increased comparably to the levels reached by insulin treatment, while the enzyme induction was not so increased. Thus, it is suggested that insulin is important in regulated translation in addition to transcription. However, triiodothyronine treatment did not have much effect on the gene expression. As a result of the present experiment, it is noted that ATP citrate-lyase-gene expression was greatly dependent on carbohydrate.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
209
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
217-22
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:1396700-ATP Citrate (pro-S)-Lyase, pubmed-meshheading:1396700-Adipose Tissue, pubmed-meshheading:1396700-Animals, pubmed-meshheading:1396700-Base Sequence, pubmed-meshheading:1396700-Blotting, Northern, pubmed-meshheading:1396700-Circadian Rhythm, pubmed-meshheading:1396700-Dietary Carbohydrates, pubmed-meshheading:1396700-Dietary Proteins, pubmed-meshheading:1396700-Fasting, pubmed-meshheading:1396700-Fructose, pubmed-meshheading:1396700-Gene Expression, pubmed-meshheading:1396700-Insulin, pubmed-meshheading:1396700-Kinetics, pubmed-meshheading:1396700-Liver, pubmed-meshheading:1396700-Male, pubmed-meshheading:1396700-Molecular Sequence Data, pubmed-meshheading:1396700-RNA, Messenger, pubmed-meshheading:1396700-Rats, pubmed-meshheading:1396700-Rats, Wistar, pubmed-meshheading:1396700-Transcription, Genetic, pubmed-meshheading:1396700-Triiodothyronine
pubmed:year
1992
pubmed:articleTitle
Effects of nutrients and hormones on gene expression of ATP citrate-lyase in rat liver.
pubmed:affiliation
Tezukayama Gakuin College, Osaka, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't