Linked Life Data

1396601

Source:http://linkedlifedata.com/resource/pubmed/id/1396601

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1992-11-19
pubmed:databankReference
pubmed:abstractText
Sec12p and Sar1p are required for the formation of transport vesicles generated from the endoplasmic reticulum (ER) in the yeast Saccharomyces cerevisiae. Sec12p is an ER type II membrane protein that mediates the membrane attachment of the GTP-binding Sar1 protein. The SAR1 gene is a multi-copy suppressor of a thermosensitive sec12 mutation. In an attempt to identify functional homologues of Sec12p and Sar1p from other eukaryotic organisms, we screened cDNA expression libraries derived from the fission yeast Schizosaccharomyces pombe and from the plant Arabidopsis thaliana for complementation of the sec12ts mutation. Four individual cDNAs were isolated, two of which encode the S. pombe and A. thaliana homologues of Sar1p. The three Sar1 proteins are 67% identical on average. The two other cDNAs encode type II membrane proteins which were designated Stl1p for the S. pombe protein and Stl2p for the A. thaliana protein (Stl stands for Sec12p-like). Both proteins have NH2-terminal cytoplasmic domains which resemble that of Sec12p: they are similar in size and present a significant degree of amino acid identity with the cytoplasmic domain of Sec12p. In contrast, the lumenal domains of Sec12p, Stl1p and Stl2p are very different in size and do not show any appreciable homology. That Stl1p and Stl2p are functional homologues of Sec12p was confirmed by showing that expression of either cloned gene complements a sec12 null mutation. Our results indicate that some of the mechanisms regulating vesicle formation at the ER are conserved not only in yeasts, but also in plants.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1096303, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1303803, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1310258, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1315316, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1537327, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1574125, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1591615, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1649197, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1680566, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1898771, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1907491, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1907973, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1907974, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1922074, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1924322, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-1944536, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2005786, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2071670, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2077687, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2077689, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2111733, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2112088, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2112089, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2123294, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2188733, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2194670, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2328721, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2501306, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2512296, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2542798, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2657434, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2659436, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-2670553, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-3047151, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-3127202, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-3162770, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-3317403, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-3390865, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-3552249, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-6394957, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-6546423, http://linkedlifedata.com/resource/pubmed/commentcorrection/1396601-7026045
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:geneSymbol
LEU2, STL1, STL2, sec12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4205-11
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
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