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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1992-11-23
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pubmed:databankReference |
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pubmed:abstractText |
The sequence element of box II (GTGTGGTTAATATG) is a regulatory component of a light-responsive element present within the upstream region of pea rbcS-3A. The nuclear protein GT-1 was defined previously as a DNA binding activity that interacts with box II. Here, we describe the isolation and characterization of cDNA sequences that encode a DNA binding protein with specificity for this element. The recombinant protein, tobacco GT-1a, shows similar sequence requirements for DNA binding to nuclear GT-1, as assayed by its ability to interact with previously defined 2-bp scanning mutations of box II, and is shown to be immunologically related to nuclear GT-1. The predicted structure of the 43-kD protein derived from the cDNA sequence suggests the presence of a novel helix-helix-turn-helix (HHTH) motif. Comparison between the predicted protein sequence encoded by the tobacco GT-1a cDNA and that of another GT binding protein, rice GT-2, reveals strong amino acid conservation over the HHTH region; this motif appears to be involved in the interaction between the recombinant protein and box II. Genomic DNA gel blot analysis indicated the presence of a small gene family of related sequences within the tobacco nuclear genome. RNA gel blot analysis of tobacco mRNA using the isolated cDNA as a probe showed that transcripts are present in several tissues, including both light-grown and dark-adapted leaves.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-1498605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-16593893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-1893099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2006188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2152164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2152170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2330508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2398902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2399285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2493990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2535506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2535518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2535536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2673023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2698648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-2890552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-3046753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-3072718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-3387433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-3678200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1392598-6345791
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1040-4651
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
839-49
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1392598-Amino Acid Sequence,
pubmed-meshheading:1392598-Base Sequence,
pubmed-meshheading:1392598-Binding Sites,
pubmed-meshheading:1392598-DNA,
pubmed-meshheading:1392598-DNA-Binding Proteins,
pubmed-meshheading:1392598-Light,
pubmed-meshheading:1392598-Molecular Sequence Data,
pubmed-meshheading:1392598-Plant Proteins,
pubmed-meshheading:1392598-Plants, Toxic,
pubmed-meshheading:1392598-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1392598-Ribulose-Bisphosphate Carboxylase,
pubmed-meshheading:1392598-Sequence Homology, Amino Acid,
pubmed-meshheading:1392598-Tobacco,
pubmed-meshheading:1392598-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
Characterization of a gene encoding a DNA binding protein with specificity for a light-responsive element.
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pubmed:affiliation |
Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10021-6399.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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