Linked Life Data

1390728

Source:http://linkedlifedata.com/resource/pubmed/id/1390728

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
39
pubmed:dateCreated
1992-11-10
pubmed:abstractText
Chemical synthesis of oligodeoxyribonucleotides modified at a preselected internucleotide bond by the replacement of one of the two "nonbridging" oxygens by a sulfur atom or an ethoxy group yields model substrates for studies on DNA-protein interactions. Chromatographic (RP-HPLC) separation of the diastereomers of oligonucleotides containing EcoRI canonical sequence together with the assignment of the substituent orientation in the DNA molecule allowed study of the stereochemical aspects of DNA-EcoRI endonuclease interactions. The DNA segment involved in interactions between EcoRI protein and phosphate groups appeared to be larger than its canonical sequence, ...GAATTC..., and was extended to the nonamer. The modification of certain internucleotide bonds within this nonamer caused significant or complete protection against the nucleolytic action of EcoRI and, in some cases, manifested the diastereoselectivity of the enzyme. On the basis of the results of EcoRI-catalyzed hydrolysis of stereodefined phosphorothioate and phosphotriester substrates, we propose a model to explain this phenomenon at the molecular level.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9460-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Application of phosphate-backbone-modified oligonucleotides in the studies on EcoRI endonuclease mechanism of action.
pubmed:affiliation
Department of Bioorganic Chemistry, Polish Academy of Sciences, Lodz.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't