Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
|
pubmed:dateCreated |
1992-12-7
|
pubmed:abstractText |
The inter-alpha-inhibitor (I alpha I) family encompasses four plasma proteins, namely free bikunin as well as I alpha I, pre-alpha-inhibitor (P alpha I) and inter-alpha-like inhibitor (I alpha LI). Each of the last three proteins is a distinct assembly of one bikunin chain with one or two out of three distinct, heavy (H) chains designated H1, H2 and H3. I alpha I is made of [H1 + H2 + bikunin], P alpha I is made of [H3 + bikunin] and I alpha LI is made of [H2 + bikunin]. We characterized various I alpha I family-related antisera/antibodies, including a reagent recognizing bikunin, another directed against the four H1, H2, H3 and bikunin chains, and a third one directed against both H1 and H2 chains. By a simplified absorption procedure of the latter two antisera onto solid-phase immunoadsorbents made with i) purified I alpha I, or ii) a recombinant H1 polypeptide, we also obtained specific anti-H3 or anti-H2 antibodies, respectively. This series of new antibodies was used to identify each component within the pattern of bands corresponding to I alpha I family in human serum, as separated by SDS-PAGE and western blotting.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alpha-Globulins,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/SPINT2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin Inhibitor, Kunitz Soybean,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/inter-alpha-inhibitor
|
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
0177-3593
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
373
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1019-24
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:1384549-Alpha-Globulins,
pubmed-meshheading:1384549-Antibodies,
pubmed-meshheading:1384549-Antibody Specificity,
pubmed-meshheading:1384549-Blotting, Western,
pubmed-meshheading:1384549-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1384549-Glycoproteins,
pubmed-meshheading:1384549-Humans,
pubmed-meshheading:1384549-Membrane Glycoproteins,
pubmed-meshheading:1384549-Molecular Weight,
pubmed-meshheading:1384549-Trypsin Inhibitor, Kunitz Soybean,
pubmed-meshheading:1384549-Trypsin Inhibitors
|
pubmed:year |
1992
|
pubmed:articleTitle |
Electrophoretic pattern of the inter-alpha-inhibitor family proteins in human serum, characterized by chain-specific antibodies.
|
pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale Unit-78, Boisguillaume, France.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|