Linked Life Data

1383329

Source:http://linkedlifedata.com/resource/pubmed/id/1383329

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1992-11-25
pubmed:abstractText
CD19 is a member of the Ig superfamily expressed on the surface of B lymphocytes that may be involved in the regulation of B cell function. Immunoprecipitation studies with B cell lines solubilized by digitonin have shown CD19 to be part of a multimolecular complex that includes CD21 (CR2) and other unidentified proteins. In this study, two of the CD19-associated proteins were identified as TAPA-1, which is expressed on most cell types, and Leu-13, which is expressed on subsets of lymphoid cells. TAPA-1 and Leu-13 are physically associated in many cell lineages. CD19 and CD21 mAb each specifically coprecipitated proteins of the same size as those precipitated by TAPA-1 and Leu-13 mAb from B cell lines and cDNA-transfected K562 cell lines. Western blot analysis with a TAPA-1 mAb verified the identity of TAPA-1 in CD19 and CD21 immunoprecipitated materials. In addition, when TAPA-1 or Leu-13 were crosslinked and patched on the cell surface, all of the CD19 comigrated with TAPA-1 and some of the CD19 comigrated with Leu-13. Furthermore, mAb binding to CD19, CD21, TAPA-1, and Leu-13 on B cell lines induced similar biologic responses, including the induction of homotypic adhesion, inhibition of proliferation, and an augmentation of the increase in intracellular [Ca2+] induced by suboptimal cross-linking of surface Ig on B cell lines. Together, these data suggest that TAPA-1 and Leu-13 are broadly expressed members of a signal transduction complex in which lineage-specific proteins, such as CD19 and CD21, provide cell-specific functions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
149
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2841-50
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:1383329-Antigens, CD, pubmed-meshheading:1383329-Antigens, CD19, pubmed-meshheading:1383329-Antigens, CD81, pubmed-meshheading:1383329-Antigens, Differentiation, pubmed-meshheading:1383329-Antigens, Differentiation, B-Lymphocyte, pubmed-meshheading:1383329-Antigens, Surface, pubmed-meshheading:1383329-B-Lymphocytes, pubmed-meshheading:1383329-Blotting, Western, pubmed-meshheading:1383329-Calcium, pubmed-meshheading:1383329-Cell Adhesion, pubmed-meshheading:1383329-Cell Line, pubmed-meshheading:1383329-Fluorescent Antibody Technique, pubmed-meshheading:1383329-Humans, pubmed-meshheading:1383329-Lymphocyte Activation, pubmed-meshheading:1383329-Membrane Proteins, pubmed-meshheading:1383329-Models, Biological, pubmed-meshheading:1383329-Receptors, Complement 3d, pubmed-meshheading:1383329-Signal Transduction, pubmed-meshheading:1383329-Transfection
pubmed:year
1992
pubmed:articleTitle
The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules.
pubmed:affiliation
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115-6084.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't