Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1992-11-12
|
pubmed:abstractText |
Neurons from the suprachiasmatic nucleus (SCN) of the hypothalamus, the site of a circadian pacemaker in mammals, were isolated from embryonic rat. After mechanical dissociation neurons were brought into culture for 1-2 weeks, using a chemically defined medium. Recordings were made from 74 bipolar neurons using two different configurations of the patch-clamp technique. During cell attached patch recordings, 45% of neurons fired spontaneously. The mean firing rate was 0.7 +/- 0.6 Hz and the firing pattern was irregular. In whole cell recordings 73% of the investigated neurons showed spontaneous activity with an irregular firing pattern. The mean spontaneous firing rate with an intracellular Cl- concentration of 145 mM was 1.0 +/- 0.6 Hz. The resting membrane potential of the bipolar neurons was estimated to be -62 +/- 24 mV. An intracellular Cl- concentration of 145 mM depolarised the membrane potential. It also increased the probability of spontaneous firing. A depolarising current stimulus produced an action potential with a threshold voltage of -46 +/- 9 mV. Suprathreshold stimuli resulted in repetitive firing with a mean frequency of 12 +/- 4 Hz. The minimum interspike interval was 52 +/- 14 ms. All action potentials either occurring spontaneously or elicited by current stimuli were abolished by the Na(+)-channel blocker TTX. These results indicate that our cultured neurons have some electrophysiological properties in common with SCN neurons in brain slices and in vivo.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0006-8993
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
14
|
pubmed:volume |
588
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
120-31
|
pubmed:dateRevised |
2003-11-14
|
pubmed:meshHeading |
pubmed-meshheading:1382804-Action Potentials,
pubmed-meshheading:1382804-Animals,
pubmed-meshheading:1382804-Cells, Cultured,
pubmed-meshheading:1382804-Chlorides,
pubmed-meshheading:1382804-Electrophysiology,
pubmed-meshheading:1382804-Female,
pubmed-meshheading:1382804-Ion Channels,
pubmed-meshheading:1382804-Isoquinolines,
pubmed-meshheading:1382804-Membrane Potentials,
pubmed-meshheading:1382804-Neurons,
pubmed-meshheading:1382804-Pregnancy,
pubmed-meshheading:1382804-Rats,
pubmed-meshheading:1382804-Rats, Inbred Strains,
pubmed-meshheading:1382804-Suprachiasmatic Nucleus
|
pubmed:year |
1992
|
pubmed:articleTitle |
Spontaneous and stimulated firing in cultured rat suprachiasmatic neurons.
|
pubmed:affiliation |
Department of Physiology, Leiden University, The Netherlands.
|
pubmed:publicationType |
Journal Article
|