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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1992-10-15
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pubmed:abstractText |
Infectivity of human T-lymphotropic virus type I (HTLV-I) to human nervous tissue cells was explored using co-cultivation with X-irradiated, HTLV-I-producing MT2 cells. Examined cells included normal cerebellar cells, brain tumor cells (astrocytoma, medulloblastoma, meningioma, hemangioblastoma, and schwannoma), and various cell lines (astrocytoma, ependymoma, oligodendroglioma, medulloblastoma, and neuroblastoma). Successful HTLV-I infection was confirmed immunohistochemically using monoclonal antibodies to HTLV-I p19, p24, and pX product. All cell lines and primary cultures from normal cerebellar tissues and brain tumors could be infected with HTLV-I. Double immunostaining showed that glial fibrillary acidic protein-, S-100 protein- or vimentin-positive cells were susceptible to infection. Neurofilament- or neuron-specific enolase-positive cells in medulloblastoma could also be infected. Reverse-transcriptase assay revealed the productive infection in U251-MG (astrocytoma) and KG-IC (oligodendroglioma) lines. Co-cultivated U251-MG cells formed syncytial polykaryons after serial passages, and polymerase chain reaction assay detected HTLV-I genome in U251-MG syncytial polykaryons and p19+ mononuclear cells. HTLV-I viral RNA was also detected in infected U251-MG cells by in situ hybridization. These data show that HTLV-I may have a wide spectrum of infectivity in human nervous tissues.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0001-6322
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
147-52
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pubmed:dateRevised |
2007-11-9
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pubmed:meshHeading |
pubmed-meshheading:1381859-Base Sequence,
pubmed-meshheading:1381859-Brain Neoplasms,
pubmed-meshheading:1381859-Cell Line,
pubmed-meshheading:1381859-DNA, Viral,
pubmed-meshheading:1381859-Human T-lymphotropic virus 1,
pubmed-meshheading:1381859-Humans,
pubmed-meshheading:1381859-Immunohistochemistry,
pubmed-meshheading:1381859-Microscopy, Electron,
pubmed-meshheading:1381859-Molecular Sequence Data,
pubmed-meshheading:1381859-Nerve Tissue,
pubmed-meshheading:1381859-Nucleic Acid Hybridization,
pubmed-meshheading:1381859-Paraparesis, Tropical Spastic,
pubmed-meshheading:1381859-Polymerase Chain Reaction,
pubmed-meshheading:1381859-RNA-Directed DNA Polymerase
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pubmed:year |
1992
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pubmed:articleTitle |
Infectivity of human T-lymphotropic virus type I to human nervous tissue cells in vitro.
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pubmed:affiliation |
Department of Pathology, Okayama University Medical School, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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