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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1992-10-8
|
| pubmed:abstractText |
Cyclic AMP (cAMP)-dependent chloride channels modulate changes in resting membrane potential and action potential duration in response to autonomic stimulation in heart. A growing body of evidence suggests that there are marked similarities in the properties of the cAMP-dependent chloride channels in heart and cystic fibrosis transmembrane regulator (CFTR) chloride channels found in airway epithelia or in cells expressing the CFTR gene product. We isolated poly A+ mRNA from rabbit ventricle and converted it to cDNA for amplification using the polymerase chain reaction (PCR). A fragment corresponding to the nucleotide-binding domain 1 (NBD1) of the CFTR transcript was cloned. Comparison of the amino acid sequence of NBD1 of human CFTR with the deduced sequence of the rabbit heart PCR product indicated 98% identity. Northern blot analysis, using the heart amplification product as a cDNA probe, demonstrated expression of homologous transcripts in human atrium, guinea pig and rabbit ventricle, and dog pancreas. Xenopus oocytes injected with poly A+ mRNA extracted from rabbit and guinea pig ventricle or dog pancreas expressed robust time-independent chloride currents in response to an elevation of cAMP. We conclude that CFTR chloride channels are expressed in heart and are responsible for the observed cAMP-dependent chloride conductance.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CFTR protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cystic Fibrosis Transmembrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0009-7330
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
71
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1002-7
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1381291-Animals,
pubmed-meshheading:1381291-Blotting, Northern,
pubmed-meshheading:1381291-Chloride Channels,
pubmed-meshheading:1381291-Chlorides,
pubmed-meshheading:1381291-Cyclic AMP,
pubmed-meshheading:1381291-Cystic Fibrosis,
pubmed-meshheading:1381291-Cystic Fibrosis Transmembrane Conductance Regulator,
pubmed-meshheading:1381291-Dogs,
pubmed-meshheading:1381291-Guinea Pigs,
pubmed-meshheading:1381291-Heart,
pubmed-meshheading:1381291-Humans,
pubmed-meshheading:1381291-Ion Channels,
pubmed-meshheading:1381291-Membrane Potentials,
pubmed-meshheading:1381291-Membrane Proteins,
pubmed-meshheading:1381291-Oocytes,
pubmed-meshheading:1381291-Polymerase Chain Reaction,
pubmed-meshheading:1381291-RNA, Messenger,
pubmed-meshheading:1381291-Rabbits,
pubmed-meshheading:1381291-Transcription, Genetic,
pubmed-meshheading:1381291-Xenopus laevis
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Expression of cystic fibrosis transmembrane regulator Cl- channels in heart.
|
| pubmed:affiliation |
Department of Physiology, University of Nevada School of Medicine, Reno 89557-0046.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|