|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1992-10-1
|
|
pubmed:abstractText |
We evaluated the effect of the antibodies to adhesion molecules CD2, CD11a/CD18 (LFA-1), and CD56 (N-CAM) on MHC-unrestricted cytotoxicity mediated by polyclonal NK cells and LAK cells or by CD3+ or CD3- cytolytic cell clones against a panel of tumor cell targets selected according to expression or absence of the corresponding ligands. We show that (i) antibodies to CD11a/CD18 and, to a lesser extent, antibodies to CD2 inhibit target cell lysis, whereas anti-CD56 antibodies exert little if any effect; (ii) in a model system using polyclonal NK/LAK cells as effectors and K562 or HL60-R (NK-resistant) cells as targets, inhibition of cytotoxicity occurs without a significant impairment of effector to target cell binding; (iii) the cytotoxic function of CD3+ or CD3- cytotoxic cell clones is inhibited differentially by antibodies to adhesion molecules; (iv) conjugates formed in the presence of antibodies which inhibit target cell lysis display a significant reduction of target to effector cell contact surface; and (v) this may lead to defective activation of effector cells, as indicated by lack of redistribution of the microtubular apparatus. We conclude that (i) MHC-unrestricted cytotoxicity is regulated by a number of molecular interactions that span far beyond our present knowledge and that it is strictly dependent on the surface phenotype of the effector cell and of the target cell; (ii) in certain types of effector/target cell interactions, antibodies to adhesion molecules do not prevent conjugate formation but reduce the extent of cell-to-cell surface contact which, in turn, leads to defective activation of the effector cell and, therefore, to inhibition of target cell lysis.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD18,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD2,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD56,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Function-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Tubulin
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0008-8749
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
143
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
N
|
|
pubmed:pagination |
389-404
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:1380897-Actins,
pubmed-meshheading:1380897-Antibodies, Monoclonal,
pubmed-meshheading:1380897-Antigens, CD,
pubmed-meshheading:1380897-Antigens, CD18,
pubmed-meshheading:1380897-Antigens, CD2,
pubmed-meshheading:1380897-Antigens, CD3,
pubmed-meshheading:1380897-Antigens, CD56,
pubmed-meshheading:1380897-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:1380897-Cell Adhesion,
pubmed-meshheading:1380897-Cell Adhesion Molecules,
pubmed-meshheading:1380897-Cells, Cultured,
pubmed-meshheading:1380897-Clone Cells,
pubmed-meshheading:1380897-Cytoskeleton,
pubmed-meshheading:1380897-Cytotoxicity, Immunologic,
pubmed-meshheading:1380897-Fluorescent Antibody Technique,
pubmed-meshheading:1380897-Humans,
pubmed-meshheading:1380897-Killer Cells, Lymphokine-Activated,
pubmed-meshheading:1380897-Killer Cells, Natural,
pubmed-meshheading:1380897-Lymphocyte Activation,
pubmed-meshheading:1380897-Lymphocyte Cooperation,
pubmed-meshheading:1380897-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:1380897-Lymphocyte Subsets,
pubmed-meshheading:1380897-Major Histocompatibility Complex,
pubmed-meshheading:1380897-Microscopy, Electron, Scanning,
pubmed-meshheading:1380897-Receptors, Antigen, T-Cell,
pubmed-meshheading:1380897-Receptors, Immunologic,
pubmed-meshheading:1380897-Tubulin
|
|
pubmed:year |
1992
|
|
pubmed:articleTitle |
Antibodies to adhesion molecules inhibit the lytic function of MHC-unrestricted cytotoxic cells by preventing their activation.
|
|
pubmed:affiliation |
Istituto di Anatomia e Istologia, Università di Genova, Italy.
|
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|
