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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1992-6-25
|
| pubmed:abstractText |
The epitope corresponding to amino acid residues 147-161 of the nucleoprotein (NP) of influenza A virus is recognized by CTL in association with H-2Kd class I Ag. Herein, we engineered an Ig molecule carrying this CTL epitope by replacing the diversity gene segment of the H chain V region of an anti-arsonate antibody with an oligonucleotide that encodes the CTL epitope. The chimeric H chain gene was expressed either alone or together with the parental L chain in the nonsecreting BALB/c myeloma B cell line, SP2/0. The Ig produced by cells transfected with both the chimeric H chain and parental L chains genes expressed the NP epitope but lost the original arsonate binding activity. In addition, SP2/0 cells expressing the chimeric H chain either alone or together with the parental L chain were lysed by class I restricted NP-epitope specific CTL. By contrast, SP2/0 cells pulsed with soluble chimeric Ig molecules were not lysed by the specific CTL. These observations indicate that: 1) this particular CTL epitope can be expressed on Ig molecules without altering the H and L chain pairing; 2) this CTL epitope can be generated from this chimeric Ig in which it is surrounded by flanking regions distinct from those of the viral NP; and 3) the generation of this CTL epitope from the Ig molecule requires the endogenous pathway as do viral proteins.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Heavy Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleocapsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Core Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
148
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3604-9
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1375250-Amino Acid Sequence,
pubmed-meshheading:1375250-Animals,
pubmed-meshheading:1375250-Antigens, Viral,
pubmed-meshheading:1375250-Base Sequence,
pubmed-meshheading:1375250-Epitopes,
pubmed-meshheading:1375250-Genes, Immunoglobulin,
pubmed-meshheading:1375250-Immunoglobulin Heavy Chains,
pubmed-meshheading:1375250-Immunoglobulin Variable Region,
pubmed-meshheading:1375250-Influenza A virus,
pubmed-meshheading:1375250-Mice,
pubmed-meshheading:1375250-Molecular Sequence Data,
pubmed-meshheading:1375250-Mutagenesis, Site-Directed,
pubmed-meshheading:1375250-Nucleocapsid Proteins,
pubmed-meshheading:1375250-Nucleoproteins,
pubmed-meshheading:1375250-Oligodeoxyribonucleotides,
pubmed-meshheading:1375250-Recombinant Fusion Proteins,
pubmed-meshheading:1375250-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:1375250-Viral Core Proteins
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Cells expressing an H chain Ig gene carrying a viral T cell epitope are lysed by specific cytolytic T cells.
|
| pubmed:affiliation |
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|