rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
1-2
|
pubmed:dateCreated |
1992-5-19
|
pubmed:abstractText |
We have developed a sensitive and specific sandwich type enzyme-linked immunosorbent assay (ELISA) to detect type IV collagenase (MMP-2) in human plasma which employs the combination of affinity purified rabbit polyclonal antibodies and mouse monoclonal antibodies to human MMP-2. The MMP-2 ELISA detects latent and activated MMP-2, MMP-2 complexed with TIMP and MMP-2 complexed with alpha 2 macroglobulin (65% efficiency). To determine whether physiologic conditions associated with increased connective tissue turnover are accompanied by increased MMP-2 levels in plasma, we compared enzyme levels in pregnant and nonpregnant women. Plasma MMP-2 (mean +/- standard deviation) was significantly increased (p less than 0.05) in the second half of pregnancy (650 +/- 312) as compared to early pregnancy (356 +/- 139) or the nonpregnant state (387 +/- 193). As a result of the linkage between type IV collagenase production by cancer cells and the metastatic phenotype, the assay of MMP-2 in plasma is of potential clinical value in cancer.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0022-1759
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
8
|
pubmed:volume |
148
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
189-98
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:1373424-Antibodies, Monoclonal,
pubmed-meshheading:1373424-Antibody Specificity,
pubmed-meshheading:1373424-Connective Tissue,
pubmed-meshheading:1373424-Dose-Response Relationship, Immunologic,
pubmed-meshheading:1373424-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1373424-Female,
pubmed-meshheading:1373424-Glycoproteins,
pubmed-meshheading:1373424-Humans,
pubmed-meshheading:1373424-Immunoblotting,
pubmed-meshheading:1373424-Matrix Metalloproteinase 9,
pubmed-meshheading:1373424-Microbial Collagenase,
pubmed-meshheading:1373424-Pregnancy,
pubmed-meshheading:1373424-Sensitivity and Specificity,
pubmed-meshheading:1373424-Tissue Inhibitor of Metalloproteinases,
pubmed-meshheading:1373424-alpha-Macroglobulins
|
pubmed:year |
1992
|
pubmed:articleTitle |
Immunoassay of type IV collagenase/gelatinase (MMP-2) in human plasma.
|
pubmed:affiliation |
Department of Research, Department of Veterans Affairs Medical Center, Northport, NY 11768.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|