Linked Life Data

1372006

Source:http://linkedlifedata.com/resource/pubmed/id/1372006

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1992-4-14
pubmed:abstractText
The recessive mutation nanomelia blocks the synthesis of a large aggregating proteoglycan (aggrecan) by avian embryo chondrocytes. Lack of aggrecan is associated with short stature, multiple morphological defects in cartilage, and embryo lethality. Bony defects have also been described, but were assumed to be a secondary consequence of the cartilage defect. However, two lines of evidence presented in this paper indicate that the aggrecan deficiency directly affects intramembranous bone. First, the morphology (i.e. projected area and shape) of certain membranous bones of nanomelia embryos was abnormal. Second, membranous bone from nanomelia embryos proved to be significantly stiffer in biomechanical tests that measured functional properties of the extracellular matrix. These findings were unexpected because intramembranous bones normally develop from mesenchyme and not from a cartilage intermediate, and they prompted a search for evidence of aggrecan expression in the bone of normal chick embryos. We report that: 1) aggrecan mRNA was identified by PCR analysis of total RNA isolated from day-13 chick embryo calvarium, 2) the PCR method successfully amplified aggrecan mRNA from primary chick embryo osteoblasts in culture, 3) in situ hybridization of membranous bone tissue sections demonstrated aggrecan expression by chick embryo osteoblasts in vivo, and 4) the aggrecan message was identified in Northern blots of calvarial mRNA probed at high stringency. The results of the molecular and biomechanical studies provide evidence that aggrecan is indeed expressed in membranous bone as well as cartilage. Altogether, these results suggest that aggrecan may contribute to the functional properties and the normal growth and development of avian membranous bone.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5592-8
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:1372006-Aggrecans, pubmed-meshheading:1372006-Animals, pubmed-meshheading:1372006-Base Sequence, pubmed-meshheading:1372006-Blotting, Northern, pubmed-meshheading:1372006-Bone and Bones, pubmed-meshheading:1372006-Cells, Cultured, pubmed-meshheading:1372006-Chick Embryo, pubmed-meshheading:1372006-Chickens, pubmed-meshheading:1372006-Chondroitin Sulfate Proteoglycans, pubmed-meshheading:1372006-Extracellular Matrix Proteins, pubmed-meshheading:1372006-Genes, Recessive, pubmed-meshheading:1372006-Lectins, C-Type, pubmed-meshheading:1372006-Molecular Sequence Data, pubmed-meshheading:1372006-Mutation, pubmed-meshheading:1372006-Oligodeoxyribonucleotides, pubmed-meshheading:1372006-Osteoblasts, pubmed-meshheading:1372006-Polymerase Chain Reaction, pubmed-meshheading:1372006-Proteoglycans, pubmed-meshheading:1372006-RNA, pubmed-meshheading:1372006-RNA, Messenger
pubmed:year
1992
pubmed:articleTitle
Aggrecan core protein is expressed in membranous bone of the chick embryo. Molecular and biomechanical studies of normal and nanomelia embryos.
pubmed:affiliation
Department of Surgery, University of Michigan, Ann Arbor 48109-0650.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't