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rdf:type |
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lifeskim:mentions |
umls-concept:C0034790,
umls-concept:C0039097,
umls-concept:C0039194,
umls-concept:C0229664,
umls-concept:C0439097,
umls-concept:C1515021,
umls-concept:C1547348,
umls-concept:C1705241,
umls-concept:C1879547,
umls-concept:C1880371,
umls-concept:C2745888
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pubmed:issue |
2
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pubmed:dateCreated |
1992-4-1
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pubmed:databankReference |
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pubmed:abstractText |
In the present study we have characterized the gamma/delta T cell receptor (TcR) population in synovial fluid (SF) and peripheral blood (PB) of patients with chronic inflammatory arthritis. By double staining we have shown that (a) synovial V delta 1+ cells have a high expression of activation markers CD45R0 ("memory cells") and HLA-DR as compared to PB, indicating a preactivated population of V delta 1-carrying T cells in vivo and (b) interleukin 2-induced expansion of synovial cells yields a high proportion of gamma/delta in most samples expressing predominantly the V delta 1 TcR. Junctional sequence analysis of the TcR delta chain from interleukin 2-expanded PB cell lines demonstrated a polyclonal V delta 1 population in three out of three samples. In SF cell lines three out of four samples were polyclonally expanded. In SF from one patient, however, a limited repertoire of expressed V delta 1 genes was found. Altogether, our data demonstrate the presence of preactivated V delta 1-expressing cells in the synovial compartment. This V delta 1 population is predominantly polyclonal, except in one patient where oligoclonally expanded V delta 1 cells were detected.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD29,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
567-74
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1371472-Antigens, CD,
pubmed-meshheading:1371472-Antigens, CD29,
pubmed-meshheading:1371472-Antigens, CD45,
pubmed-meshheading:1371472-Arthritis, Rheumatoid,
pubmed-meshheading:1371472-Base Sequence,
pubmed-meshheading:1371472-Flow Cytometry,
pubmed-meshheading:1371472-Gene Rearrangement, delta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:1371472-HLA-DR Antigens,
pubmed-meshheading:1371472-Histocompatibility Antigens,
pubmed-meshheading:1371472-Humans,
pubmed-meshheading:1371472-Lymphocyte Activation,
pubmed-meshheading:1371472-Molecular Sequence Data,
pubmed-meshheading:1371472-Oligodeoxyribonucleotides,
pubmed-meshheading:1371472-Polymerase Chain Reaction,
pubmed-meshheading:1371472-RNA, Messenger,
pubmed-meshheading:1371472-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:1371472-Synovial Fluid,
pubmed-meshheading:1371472-T-Lymphocyte Subsets
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pubmed:year |
1992
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pubmed:articleTitle |
T cell receptor diversity and activation markers in the V delta 1 subset of rheumatoid synovial fluid and peripheral blood T lymphocytes.
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pubmed:affiliation |
Department of Pharmacology, Kabi Pharmacia AB, Autoimmunity, Uppsala, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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