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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1992-12-7
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pubmed:abstractText |
Calf thymus proliferating cell nuclear antigen (PCNA) promoted DNA synthesis past cis-syn and trans-syn-I cyclobutane thymine dimers by calf thymus DNA polymerase delta (Pol delta) in vitro. Templates containing site-specific cis-syn and trans-syn-I thymine dimers were prepared via a combination of solid phase synthesis with photoproduct building blocks and DNA ligation. Extension of a 15-mer primer on the UV dimer-containing templates by Pol delta produced termination and bypass products in a dNTP and PCNA dependent manner. In the absence of PCNA and at dNTP concentrations varying between 1 and 100 microM, Pol delta could not bypass the cis-syn dimer and terminated elongation one nucleotide prior to the 3'-T of the dimer. DNA synthesis past the trans-syn-I dimer was even less efficient. In the presence of PCNA, termination occurred primarily one nucleotide prior to the 3'-T of both dimers at 1 microM dNTPs but opposite the 5'-T of the dimers at 100 microM dNTPs. In addition, under the latter conditions, bypass of the dimers was observed, to the extent of about 30% of the products for the cis-syn dimer and about 15% for the trans-syn-I dimer.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-1349852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-1682321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-1924370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-1967833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-2185842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-2271562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-2431928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-2552436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-2670563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-2676986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-2825983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-2894900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-3025594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-3048386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-3323527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-3387219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-3745189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-3763398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1359505-6426510
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5403-6
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1359505-Animals,
pubmed-meshheading:1359505-Antigens, Neoplasm,
pubmed-meshheading:1359505-Autoantigens,
pubmed-meshheading:1359505-Base Sequence,
pubmed-meshheading:1359505-Cattle,
pubmed-meshheading:1359505-DNA Polymerase III,
pubmed-meshheading:1359505-DNA Replication,
pubmed-meshheading:1359505-DNA-Directed DNA Polymerase,
pubmed-meshheading:1359505-Molecular Sequence Data,
pubmed-meshheading:1359505-Nuclear Proteins,
pubmed-meshheading:1359505-Oligodeoxyribonucleotides,
pubmed-meshheading:1359505-Proliferating Cell Nuclear Antigen,
pubmed-meshheading:1359505-Pyrimidine Dimers,
pubmed-meshheading:1359505-Thymus Gland
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pubmed:year |
1992
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pubmed:articleTitle |
PCNA-induced DNA synthesis past cis-syn and trans-syn-I thymine dimers by calf thymus DNA polymerase delta in vitro.
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pubmed:affiliation |
Department of Chemistry, Washington University, St Louis, MO 63130.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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