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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1992-9-14
|
| pubmed:abstractText |
Complete elimination of CD4 cells by in vivo treatment with anti-CD4 mAb may result in B cell polyclonal activation. Additionally, mice treated with doses of anti-CD4 that eliminate half the CD4 cells produced higher anti-SRBC antibody responses than controls. This suggests that partial CD4 depletion enhances Th2-like function. To test this hypothesis we examined Th1 and Th2 lymphokine potential in mice partially depleted of CD4 cells. We measured IL-4 and IFN-gamma secretion by stimulated unfractionated spleen cells and analyzed activated, purified CD4 cells by RNA in situ hybridization to determine the percentage of IFN-gamma- or IL-4-producing cells. Unfractionated splenocytes from partially CD4-depleted mice secreted more IL-4 and less IFN-gamma than splenocytes from control mice. In situ hybridization proved that CD4 cells from partially depleted mice contained a higher percentage of IL-4 and a lower percentage of IFN-gamma-producing cells than controls. These results indicate that treatment with a dose of mAb resulting in partial CD4 depletion may permit increased Th2-like lymphokine expression. This study also provides evidence that cells committed to Th2-like function exist in vivo in mice.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
149
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1131-7
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1354229-Animals,
pubmed-meshheading:1354229-Antibodies, Monoclonal,
pubmed-meshheading:1354229-Antibody Formation,
pubmed-meshheading:1354229-CD4-CD8 Ratio,
pubmed-meshheading:1354229-CD4-Positive T-Lymphocytes,
pubmed-meshheading:1354229-Dose-Response Relationship, Immunologic,
pubmed-meshheading:1354229-Female,
pubmed-meshheading:1354229-Gene Expression,
pubmed-meshheading:1354229-Interferon-gamma,
pubmed-meshheading:1354229-Interleukin-4,
pubmed-meshheading:1354229-Lymphocyte Activation,
pubmed-meshheading:1354229-Lymphocyte Cooperation,
pubmed-meshheading:1354229-Lymphocyte Depletion,
pubmed-meshheading:1354229-Mice,
pubmed-meshheading:1354229-Mice, Inbred DBA,
pubmed-meshheading:1354229-RNA, Messenger,
pubmed-meshheading:1354229-T-Lymphocyte Subsets,
pubmed-meshheading:1354229-T-Lymphocytes, Helper-Inducer
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Altered IFN-gamma and IL-4 pattern lymphokine secretion in mice partially depleted of CD4 T cells by anti-CD4 monoclonal antibody.
|
| pubmed:affiliation |
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|