Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
1992-9-23
pubmed:abstractText
A Trypanosoma cruzi expression vector has been constructed using sequences derived from the flanking regions of the glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) genes. The neomycin phosphotransferase (neor) gene was incorporated as a selectable marker. Using electroporation we have introduced this vector into both T. cruzi and Leishmania cells and conferred G418 resistance. Transformation is mediated by large extrachromosomal circular elements composed of head-to-tail tandem repeats of the vector. The transformed phenotype is stable for at least 6 months in the absence of G418 and can be maintained during passage through the T. cruzi life-cycle. Foreign genes inserted into an expression site within the vector (pTEX) can be expressed at high levels in transformed cells. To our knowledge this paper describes the first trypanosome shuttle vector and the first vector which functions in both trypanosomes and Leishmania.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-15462837, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-1656255, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-1694012, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-1697265, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-1710026, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-1713688, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-1840624, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-1906580, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-1986230, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-1992478, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-2006150, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-2124701, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-2167831, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-2172836, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-2177225, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-2234081, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-2300209, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-2304458, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-2342468, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-2594753, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-2822260, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-2972929, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-3221866, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-3366120, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-3538420, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-6273866, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-6489338, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-6960240, http://linkedlifedata.com/resource/pubmed/commentcorrection/1324472-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
20
pubmed:geneSymbol
gGAPDH
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3963-9
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
A shuttle vector which facilitates the expression of transfected genes in Trypanosoma cruzi and Leishmania.
pubmed:affiliation
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't