Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-7-15
pubmed:abstractText
When cells from the cerebellum of late-gestation rats were grown at low density (50-100 cells/mm2) in a three-dimensional culture system, they rapidly expressed morphological and electrophysiological properties of neurons. Growth and differentiation of the population of solitary neurons as a whole was statistically assessed at 6, 12, 18, 24, 48 and 72 h using data obtained from image analysis. Mean length of axons and dendrites increased 17- and 220-fold, respectively, from 6 to 72 h. Average number of branch points rose 35-fold. Other indices of complexity increased 2- to 3-fold. Whole-cell voltage clamp revealed that as early as 24-30 h the cells displayed a tetrodotoxin-sensitive Na+ current (INa), a nifedipine-sensitive high-threshold Ca2+ current, a Ni(2+)-sensitive low-threshold Ca2+ current, and two voltage-dependent K+ outward currents consisting of a 4-aminopyridine-sensitive fast transient outward current and a CsCl-sensitive slow delayed component. These observations correlate closely with voltage-activated currents previously recorded in neonatal or young rat cerebellum, and demonstrate that the culture model is useful for analyzing the early rapid growth, differentiation and intrinsic ionic currents of these neurons as single cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0378-5866
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-43
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1318190-Action Potentials, pubmed-meshheading:1318190-Animals, pubmed-meshheading:1318190-Axons, pubmed-meshheading:1318190-Calcium Channels, pubmed-meshheading:1318190-Cell Differentiation, pubmed-meshheading:1318190-Cell Division, pubmed-meshheading:1318190-Cerebellar Nuclei, pubmed-meshheading:1318190-Cesium, pubmed-meshheading:1318190-Chlorides, pubmed-meshheading:1318190-Culture Techniques, pubmed-meshheading:1318190-Dendrites, pubmed-meshheading:1318190-Electrophysiology, pubmed-meshheading:1318190-Ion Channel Gating, pubmed-meshheading:1318190-Neurons, pubmed-meshheading:1318190-Nifedipine, pubmed-meshheading:1318190-Potassium Channels, pubmed-meshheading:1318190-Rats, pubmed-meshheading:1318190-Rats, Inbred Strains, pubmed-meshheading:1318190-Sodium Channels, pubmed-meshheading:1318190-Tetrodotoxin
pubmed:year
1992
pubmed:articleTitle
Utilization of three-dimensional culture for early morphometric and electrophysiological analyses of solitary cerebellar neurons.
pubmed:affiliation
Department of Cell Biology and Anatomy, Texas Tech University Health Sciences Center, School of Medicine, Lubbock 79430.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't