Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1992-6-25
pubmed:abstractText
The Ag receptor (TCR) on T lymphocytes has been shown to be specific for foreign antigenic peptides bound to MHC-encoded molecules. During T cell differentiation in the thymus this same TCR mediates the recognition of MHC molecules in the absence of foreign Ag, a process termed positive selection. To analyze the structural relationship between MHC-restricted Ag recognition and positive selection, we characterized two different transgenic lines of mice bearing TCR specific for pigeon cytochrome c and the Ek class II MHC molecule. The two TCR expressed in these animals differed by only one amino acid in the V-J junction of the alpha-chain. In vitro, we find that this TCR difference alters Ag fine specificity. Analysis of transgenic animals demonstrates that this change in the putative third complementarity determining region of the TCR also alters the specificity of positive selection in the thymus. These results suggest that the diversity of a TCR region that can be shown to affect the specificity of foreign Ag recognition may be influenced by selection in the thymus. The findings presented here are discussed in relation to the possible role of self-peptides in positive selection.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
148
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3342-53
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1316916-Amino Acid Sequence, pubmed-meshheading:1316916-Animals, pubmed-meshheading:1316916-Autoantigens, pubmed-meshheading:1316916-Bone Marrow Cells, pubmed-meshheading:1316916-Cytochrome c Group, pubmed-meshheading:1316916-Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor, pubmed-meshheading:1316916-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, pubmed-meshheading:1316916-Lymph Nodes, pubmed-meshheading:1316916-Lymphocyte Activation, pubmed-meshheading:1316916-Major Histocompatibility Complex, pubmed-meshheading:1316916-Mice, pubmed-meshheading:1316916-Mice, Transgenic, pubmed-meshheading:1316916-Molecular Sequence Data, pubmed-meshheading:1316916-Peptides, pubmed-meshheading:1316916-Radiation Chimera, pubmed-meshheading:1316916-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:1316916-Thymus Gland
pubmed:year
1992
pubmed:articleTitle
Involvement of the same region of the T cell antigen receptor in thymic selection and foreign peptide recognition.
pubmed:affiliation
Department of Immunology, Scripps Research Institute, La Jolla, CA 92037.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't