Linked Life Data

1316367

Source:http://linkedlifedata.com/resource/pubmed/id/1316367

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1992-6-17
pubmed:abstractText
The main parameters of immunostaining techniques, i.e., the type of fixative, immunocytochemical reaction, and quality of monoclonal antibodies (MAbs), for quantitation of human cytomegalovirus (HCMV) antigenemia in peripheral blood polymorphonuclear leukocytes (currently performed by the indirect immunofluorescence or immunoperoxidase reaction by using MAbs to HCMV pp65) were investigated in order to optimize procedural steps and reagents. Significantly better results (in terms of the number of positive cells) were obtained on multiple cytospin preparations from heart transplant recipients with HCMV viremia when we used (i) formalin instead of methanol-acetone fixation and (ii) the indirect immunofluorescence reaction instead of the immunoperoxidase reaction, the avidin-biotin complex method, or the alkaline phosphatase antialkaline phosphatase procedure. In addition, comparison of the staining capabilities of three MAbs to pp65, which were developed in the laboratory and which were reactive to different epitopes of the protein, with a commercially available MAb (Clonab CMV) for determination of HCMV antigenemia showed that, while individual MAbs did not provide better results, the pool of MAbs detected a significantly higher number of positive peripheral blood polymorphonuclear leukocytes than Clonab CMV did. In addition, the sensitivity of the pool in detecting patients with low levels of viremia (less than 5/2 x 10(5) cells inoculated) as antigenemia positive was 100%, whereas the sensitivity of Clonab CMV was 47%. No differences in the specificities between the two MAb preparations were observed.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-1311365, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-1647404, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-1651361, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-1667791, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-1849539, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-2175616, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-2177748, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-2543708, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-2547818, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-2549101, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-2557386, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-2839611, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-2852670, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-3020158, http://linkedlifedata.com/resource/pubmed/commentcorrection/1316367-6318633
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1232-7
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia.
pubmed:affiliation
Institute of Infectious Diseases, Policlinico S. Matteo, University of Pavia, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't