Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-6-16
pubmed:abstractText
Exclusion and inclusion of T cell receptor (TCR) genes were analyzed in alpha beta TCR transgenic mice. Both transgenes are expressed unusually early on the surface of CD4-8-, HSA+, IL-2R- thymocytes. These progenitor cells give rise to progeny, which at the single-cell level contains endogenous alpha but not beta TCR-RNA as well as protein, in addition to products encoded by the transgenes. Thus, the surface expression of an alpha beta TCR does not prevent further alpha TCR rearrangement in immature thymocytes that still transcribe RAG-1 and RAG-2 genes. Reduced levels of RAG-1 and RAG-2 RNA are detectable only in CD4+8+ TCR high cells, which result from positive selection in the thymus. The results suggest that a developing T cell may try different alpha beta TCRs for binding to thymic MHC ligands, and that recombination at the alpha locus ceases only after positive selection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
69
pubmed:geneSymbol
RAG-1, RAG-2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
529-37
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1316241-Alleles, pubmed-meshheading:1316241-Animals, pubmed-meshheading:1316241-Base Sequence, pubmed-meshheading:1316241-DNA-Binding Proteins, pubmed-meshheading:1316241-Flow Cytometry, pubmed-meshheading:1316241-Gene Expression, pubmed-meshheading:1316241-Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor, pubmed-meshheading:1316241-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, pubmed-meshheading:1316241-Genes, pubmed-meshheading:1316241-Homeodomain Proteins, pubmed-meshheading:1316241-Mice, pubmed-meshheading:1316241-Mice, Inbred C57BL, pubmed-meshheading:1316241-Mice, Transgenic, pubmed-meshheading:1316241-Molecular Sequence Data, pubmed-meshheading:1316241-Oligodeoxyribonucleotides, pubmed-meshheading:1316241-Proteins, pubmed-meshheading:1316241-RNA, Messenger, pubmed-meshheading:1316241-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:1316241-T-Lymphocytes, pubmed-meshheading:1316241-Thymus Gland
pubmed:year
1992
pubmed:articleTitle
Exclusion and inclusion of alpha and beta T cell receptor alleles.
pubmed:affiliation
Basel Institute for Immunology, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't