Linked Life Data

1314784

Source:http://linkedlifedata.com/resource/pubmed/id/1314784

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-5-28
pubmed:abstractText
In contrast to the strong association of human papillomavirus (HPV) type 16 with genital malignancies, HPV 6 has been found essentially in benign genital lesions. In these studies we show that HPV type 6 and 16 DNAs behave differently also in their ability to transform NIH 3T3 cells in cooperation with the carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Although we could show that both HPV-6- and HPV-16-transfected genomes were integrated and expressed in NIH 3T3 cells, only the NIH 3T3 cells which contained the HPV 16 genome became fully transformed after MNNG treatment, as assessed by their ability to form colonies in soft agar and to induce tumors in nude mice. NIH 3T3 cells containing the HPV 6 genome and treated with MNNG did not show this potential. Furthermore, we could detect an increased expression of the E7 gene of HPV 16 in the carcinogen-treated cells containing the HPV 16 genome. These studies indicate that the presence of the HPV 16 genome specifically is also an essential step to in vitro cellular transformation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0300-5526
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
76-85
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Differential cooperation of a carcinogen with human papillomavirus type 6 and 16 DNAs in in vitro oncogenic transformation.
pubmed:affiliation
Unit for Development of Molecular Biology and Genetic Engineering, Hadassah University Hospital, Jerusalem, Israel.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't