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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-5-20
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pubmed:databankReference |
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pubmed:abstractText |
A human BK-2 bradykinin receptor was cloned from the lung fibroblast cell line CCD-16Lu. The cDNA clone encodes a 364 amino acid protein that has the characteristics of a seven transmembrane domain G-protein coupled receptor. The predicted amino acid sequence of the human BK-2 receptor is 81% identical to the smooth muscle rat BK-2 receptor (1). Transfection of the human BK-2 receptor cDNA into COS-7 cells results in the expression of high levels of specific BK binding sites. Saturation binding analysis indicates that the human BK-2 receptor expressed in COS-7 cells binds BK with a KD of 0.13 nM. Pharmacological characterization of the expressed BK receptor is consistent with the cDNA encoding a receptor of the BK-2 subtype. The BK-2 receptor antagonist Hoe 140 (2), D-Arg0[Hyp3, Thi5, D-Tic7, Oic8]BK has a high affinity (IC50 = 65 pM) for the cloned human receptor. The tissue distribution of the human BK-2 receptor was analyzed by competitive PCR with human tissue cDNA and is similar to that determined for the BK-2 receptor in the rat.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
184
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
260-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1314587-Amino Acid Sequence,
pubmed-meshheading:1314587-Animals,
pubmed-meshheading:1314587-Base Sequence,
pubmed-meshheading:1314587-Binding, Competitive,
pubmed-meshheading:1314587-Bradykinin,
pubmed-meshheading:1314587-Cell Line,
pubmed-meshheading:1314587-Cell Membrane,
pubmed-meshheading:1314587-Cloning, Molecular,
pubmed-meshheading:1314587-Glycosylation,
pubmed-meshheading:1314587-Humans,
pubmed-meshheading:1314587-Kinetics,
pubmed-meshheading:1314587-Lung,
pubmed-meshheading:1314587-Molecular Sequence Data,
pubmed-meshheading:1314587-Oligodeoxyribonucleotides,
pubmed-meshheading:1314587-Polymerase Chain Reaction,
pubmed-meshheading:1314587-Protein Kinases,
pubmed-meshheading:1314587-RNA, Messenger,
pubmed-meshheading:1314587-Rats,
pubmed-meshheading:1314587-Receptors, Bradykinin,
pubmed-meshheading:1314587-Receptors, Neurotransmitter,
pubmed-meshheading:1314587-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1314587-Transfection
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pubmed:year |
1992
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pubmed:articleTitle |
Cloning and pharmacological characterization of a human bradykinin (BK-2) receptor.
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pubmed:affiliation |
Merck Research Laboratories, Department of Molecular Pharmacology and Biochemistry, Rahway, N.J. 07065.
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pubmed:publicationType |
Journal Article,
Comparative Study
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