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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4 Pt 1
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pubmed:dateCreated |
1992-5-21
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pubmed:abstractText |
O2-induced impairment of mitochondrial energy generation was examined in intact lungs isolated from rats after 18-30 h exposure to either air or 100% O2 in vivo. Mitochondrial metabolic rates were determined by separate measurements of 14CO2 production from [1-14C]pyruvate and [U-14C]palmitate, perfused under normal and stimulated metabolic conditions brought about by perfusion with the uncoupler of oxidative phosphorylation, 2,4-dinitrophenol (DNP). In the absence of DNP, O2 exposure did not significantly alter 14CO2 productions from either substrate. DNP increased lung pyruvate and palmitate catabolism to CO2 twofold in air-exposed lungs but did not alter 14CO2 production in lungs isolated from O2-exposed rats. These data demonstrated an O2-induced impairment of maximal mitochondrial metabolism of both pyruvate and palmitate that could not be explained by alterations in tissue free coenzyme A or by loss of pyridine nucleotides. However, comparisons of the steady-state levels of tricarboxylic acid cycle intermediates between O2- and air-exposed lungs did identify isocitrate dehydrogenase as a possible site of O2-induced enzyme inactivation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5'-Guanylic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Adenine Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Coenzyme A,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Ketoglutaric Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen,
http://linkedlifedata.com/resource/pubmed/chemical/Palmitic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Palmitic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Pyruvates,
http://linkedlifedata.com/resource/pubmed/chemical/Pyruvic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-ketoglutaric acid
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0002-9513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
262
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
L495-501
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1314504-5'-Guanylic Acid,
pubmed-meshheading:1314504-Adenine Nucleotides,
pubmed-meshheading:1314504-Animals,
pubmed-meshheading:1314504-Citric Acid Cycle,
pubmed-meshheading:1314504-Coenzyme A,
pubmed-meshheading:1314504-Guanosine Diphosphate,
pubmed-meshheading:1314504-Guanosine Triphosphate,
pubmed-meshheading:1314504-Ketoglutaric Acids,
pubmed-meshheading:1314504-Lung,
pubmed-meshheading:1314504-Male,
pubmed-meshheading:1314504-Oxygen,
pubmed-meshheading:1314504-Palmitic Acid,
pubmed-meshheading:1314504-Palmitic Acids,
pubmed-meshheading:1314504-Pulmonary Circulation,
pubmed-meshheading:1314504-Pyruvates,
pubmed-meshheading:1314504-Pyruvic Acid,
pubmed-meshheading:1314504-Rats,
pubmed-meshheading:1314504-Rats, Inbred Strains
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pubmed:year |
1992
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pubmed:articleTitle |
Tricarboxylic acid cycle activity in perfused rat lungs after O2 exposure.
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pubmed:affiliation |
Division of Physiology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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