Linked Life Data

1313808

Source:http://linkedlifedata.com/resource/pubmed/id/1313808

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1992-5-12
pubmed:databankReference
pubmed:abstractText
The cellular retinoic acid-binding protein type II (CRABP-II) is a member of the serum and cytoplasmic retinoid-binding protein family. It is expressed during embryonic development and in adult skin and is upregulated by retinoic acid (RA) in F9 teratocarcinoma cells. We have determined the genomic organization of the murine CRABP-II gene and performed a detailed analysis of its transcriptional unit. The CRABP-II gene, located on mouse chromosome 2, is approximately 4.6 kilobases long and divided into four exons in a structure common to other members of the family of serum and cellular retinoid-binding proteins. Primer extension analysis and S1 nuclease protection assay were used to identify the transcription initiation site which is located 27 base pairs downstream of a typical TATAA box. Sequence analysis of the promoter also revealed a GC-rich region with overlapping putative SP1-binding sites at nucleotides -61 and AP-1 and AP-2-binding sites at nucleotides -518 and -544, respectively. The 3'-untranslated region contains two copies of the pentanucleotide AUUUA shown to be involved in messenger RNA destabilization. Consensus sequence for retinoic acid response elements were not detected in the promoter region of the CRABP-II gene. Results of nuclear run on experiments show that the CRABP-II gene is not transcriptionally activated by RA in F9 teratocarcinoma cells. These results suggest that the up-regulation of CRABP-II mRNA levels by RA is mainly controlled by a post-transcriptional mechanism.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
267
pubmed:geneSymbol
CRABP-II
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7777-83
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1313808-Amino Acid Sequence, pubmed-meshheading:1313808-Animals, pubmed-meshheading:1313808-Base Sequence, pubmed-meshheading:1313808-Carrier Proteins, pubmed-meshheading:1313808-Chloramphenicol O-Acetyltransferase, pubmed-meshheading:1313808-Chromosome Mapping, pubmed-meshheading:1313808-Cloning, Molecular, pubmed-meshheading:1313808-Cycloheximide, pubmed-meshheading:1313808-DNA, pubmed-meshheading:1313808-Female, pubmed-meshheading:1313808-Gene Expression Regulation, pubmed-meshheading:1313808-Mice, pubmed-meshheading:1313808-Mice, Inbred C57BL, pubmed-meshheading:1313808-Molecular Sequence Data, pubmed-meshheading:1313808-Promoter Regions, Genetic, pubmed-meshheading:1313808-RNA, Messenger, pubmed-meshheading:1313808-RNA Processing, Post-Transcriptional, pubmed-meshheading:1313808-Receptors, Retinoic Acid, pubmed-meshheading:1313808-Teratoma, pubmed-meshheading:1313808-Transcription, Genetic, pubmed-meshheading:1313808-Tretinoin, pubmed-meshheading:1313808-Tumor Cells, Cultured
pubmed:year
1992
pubmed:articleTitle
The murine gene for cellular retinoic acid-binding protein type II. Genomic organization, chromosomal localization, and post-transcriptional regulation by retinoic acid.
pubmed:affiliation
Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't