Linked Life Data

13129602

Source:http://linkedlifedata.com/resource/pubmed/id/13129602

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-9-17
pubmed:abstractText
We constructed a stable, low-copy-number plasmid containing a fusion between a Sinorhizobium meliloti rRNA promoter and gfp(mut3). When transformed into S. meliloti the resulting strain, Rm1021/pKW1, fluoresced in proportion to its growth rate during balanced growth. This strain also showed an unexpected behavior when grown to stationary phase in TY medium: the average cellular fluorescence increased through mid-exponential phase then decreased dramatically. The explanation for this appears to be that transcription from the rRNA promoter was shut off in mid-exponential phase and intracellular Gfp was diluted by continued cell growth.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0378-1097
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
226
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-22
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Transcriptional control of a rRNA promoter of the nodulating symbiont Sinorhizobium meliloti.
pubmed:affiliation
University of Connecticut, Department of Molecular and Cell Biology, 91 N. Eagleville Rd., U-44, Storrs, CT 06269, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't