Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1992-4-16
|
| pubmed:abstractText |
The genomic RNA of hepatitis C virus (HCV) in the plasma of volunteer blood donors was detected by using the polymerase chain reaction in a fraction of density 1.08 g/ml from sucrose density gradient equilibrium centrifugation. When the fraction was treated with the detergent NP40 and recentrifuged in sucrose, the HCV RNA banded at 1.25 g/ml. Assuming that NP40 removed a lipid-rich surface coat from HCV, the 1.08 g/ml and 1.25 g/ml HCV RNA may correspond to intact HCV virions and nucleocapsids, respectively. The extraordinarily low density of the virion is unusual in comparison to the density of classified viruses.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0022-1317
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
73 ( Pt 3)
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
715-8
|
| pubmed:dateRevised |
2001-11-2
|
| pubmed:meshHeading |
pubmed-meshheading:1312126-Base Sequence,
pubmed-meshheading:1312126-Blood Donors,
pubmed-meshheading:1312126-Capsid,
pubmed-meshheading:1312126-Centrifugation, Density Gradient,
pubmed-meshheading:1312126-Hepacivirus,
pubmed-meshheading:1312126-Molecular Sequence Data,
pubmed-meshheading:1312126-Polymerase Chain Reaction,
pubmed-meshheading:1312126-RNA, Viral,
pubmed-meshheading:1312126-Virion
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Extraordinarily low density of hepatitis C virus estimated by sucrose density gradient centrifugation and the polymerase chain reaction.
|
| pubmed:affiliation |
Japanese Red Cross Blood Center, Saitama.
|
| pubmed:publicationType |
Journal Article
|