Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-3-16
pubmed:abstractText
The simian virus 40 small-t antigen contains 10 cysteine residues, 6 of which are organized in two CysXCysXXCys clusters. Mutation of individual Cys residues in the two clusters or mutation of specific residues found between these clusters causes pronounced instability of the protein in animal cells. Protein instability correlates with failure of the bacterially expressed mutant proteins to bind zinc ions, an interaction which allows purification of large amounts of small-t antigen in monomeric form.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-1706474, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-1848668, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-210390, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-2107541, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-2153055, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-2157202, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-2159550, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-2170691, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-2535757, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-2536841, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-2828672, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-2969056, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-2993663, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-3260031, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-3283939, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-6261013, http://linkedlifedata.com/resource/pubmed/commentcorrection/1310775-6302673
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1746-51
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Effect of zinc ions on the biochemical behavior of simian virus 40 small-t antigen expressed in bacteria.
pubmed:affiliation
Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois 60611.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't