Linked Life Data

1310281

Source:http://linkedlifedata.com/resource/pubmed/id/1310281

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-3-3
pubmed:abstractText
Treatment of anterior pituitary reaggregate cell cultures from 14-day-old female rats with physiological doses of the gonadotropin-releasing hormone LHRH or neuropeptide Y (NPY) for 40 h dose-dependently increased [3H]thymidine ([3H]T) incorporation into DNA of cells expressing PRL immunoreactivity (PRL-ir) and of those expressing ACTH-ir, whereas these peptides decreased the number of [3H]T-labeled cells expressing GH-ir. The effects of NPY were of the same magnitude as those of LHRH. The effects of LHRH were not seen in a gonadotroph-deprived cell population obtained by sequential velocity and buoyant density gradient sedimentation. When the latter cell population was coaggregated with purified gonadotrophs from 14-day-old rats, LHRH did enhance [3H]T labeling of lactotrophs and decreased that of somatotrophs. Gonadotroph-conditioned medium obtained by continuous perifusion of gonadotroph-rich reaggregates contained four different high molecular weight substances mimicking the effects of LHRH and NPY on [3H]T incorporation in the respective pituitary cell types. These substances were partially purified and separated from each other by concentration on a Bond-elut C18-reversed phase cartridge, ultrafiltration, and C18-reversed phase HPLC. One factor stimulated [3H]T labeling of lactotrophs, another that of corticotrophs, and two others inhibited [3H]T labeling of somatotrophs. The present data suggest that the development of PRL-, GH-, and ACTH-containing cells in the pituitary is modulated by LHRH and/or NPY and that the action of LHRH and probably also of NPY is mediated by specific paracrine growth factors released from gonadotrophs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
130
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
882-94
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1310281-Adrenocorticotropic Hormone, pubmed-meshheading:1310281-Animals, pubmed-meshheading:1310281-Autoradiography, pubmed-meshheading:1310281-Cell Separation, pubmed-meshheading:1310281-Cells, Cultured, pubmed-meshheading:1310281-Centrifugation, Density Gradient, pubmed-meshheading:1310281-DNA, pubmed-meshheading:1310281-DNA Replication, pubmed-meshheading:1310281-Dose-Response Relationship, Drug, pubmed-meshheading:1310281-Female, pubmed-meshheading:1310281-Gonadotropin-Releasing Hormone, pubmed-meshheading:1310281-Growth Hormone, pubmed-meshheading:1310281-Growth Substances, pubmed-meshheading:1310281-Kinetics, pubmed-meshheading:1310281-Mitotic Index, pubmed-meshheading:1310281-Neuropeptide Y, pubmed-meshheading:1310281-Pituitary Gland, Anterior, pubmed-meshheading:1310281-Prolactin, pubmed-meshheading:1310281-Rats, pubmed-meshheading:1310281-Rats, Inbred Strains, pubmed-meshheading:1310281-Thymidine, pubmed-meshheading:1310281-Time Factors, pubmed-meshheading:1310281-Tritium
pubmed:year
1992
pubmed:articleTitle
Luteinizing hormone-releasing hormone and neuropeptide Y influence deoxyribonucleic acid replication in three anterior pituitary cell types. Evidence for mediation by growth factors released from gonadotrophs.
pubmed:affiliation
Laboratory of Cell Pharmacology, University of Leuven, School of Medicine, Belgium.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't