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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0019134,
umls-concept:C0380603,
umls-concept:C0597357,
umls-concept:C1156237,
umls-concept:C1167622,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1749467
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pubmed:issue |
1
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pubmed:dateCreated |
1992-2-10
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pubmed:abstractText |
Heparin is required for the binding of basic fibroblast growth factor (bFGF) to high-affinity receptors on cells deficient in cell surface heparan sulfate proteoglycan. So that this heparin requirement could be evaluated in the absence of other cell surface molecules, we designed a simple assay based on a genetically engineered soluble form of murine FGF receptor 1 (mFR1) tagged with placental alkaline phosphatase. Using this assay, we showed that FGF-receptor binding has an absolute requirement for heparin. By using a cytokine-dependent lymphoid cell line engineered to express mFR1, we also showed that FGF-induced mitogenic activity is heparin dependent. Furthermore, we tested a series of small heparin oligosaccharides of defined lengths for their abilities to support bFGF-receptor binding and biologic activity. We found that a heparin oligosaccharide with as few as eight sugar residues is sufficient to support these activities. We also demonstrated that heparin facilitates FGF dimerization, a property that may be important for receptor activation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-1646484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-1698555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-1707542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-1828890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-1840698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-1847668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-1848667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-1856216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-1996955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2001586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2017164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2083085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2144898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2158859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2440668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2541764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2553717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2590941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2668264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2708348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2831066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2832850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2843546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2933029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2944511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2954157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-2965305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-3025637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-3032990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-3142878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-3263970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-3417148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-3470794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-3924409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1309590-6192498
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
240-7
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1309590-3T3 Cells,
pubmed-meshheading:1309590-Alkaline Phosphatase,
pubmed-meshheading:1309590-Amino Acid Sequence,
pubmed-meshheading:1309590-Animals,
pubmed-meshheading:1309590-Base Sequence,
pubmed-meshheading:1309590-Cell Division,
pubmed-meshheading:1309590-Cell-Free System,
pubmed-meshheading:1309590-DNA,
pubmed-meshheading:1309590-Fibroblast Growth Factor 2,
pubmed-meshheading:1309590-Heparin,
pubmed-meshheading:1309590-Humans,
pubmed-meshheading:1309590-Mice,
pubmed-meshheading:1309590-Molecular Sequence Data,
pubmed-meshheading:1309590-Oligosaccharides,
pubmed-meshheading:1309590-Receptors, Cell Surface,
pubmed-meshheading:1309590-Receptors, Fibroblast Growth Factor,
pubmed-meshheading:1309590-Solubility
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pubmed:year |
1992
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pubmed:articleTitle |
Heparin is required for cell-free binding of basic fibroblast growth factor to a soluble receptor and for mitogenesis in whole cells.
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pubmed:affiliation |
Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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