Linked Life Data

1299267

Source:http://linkedlifedata.com/resource/pubmed/id/1299267

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1993-5-20
pubmed:abstractText
Fragments of rough endoplasmic reticulum or Golgi complex isolated from normal adult rat liver homogenates were injected into one cell of cleaving two-cell Xenopus laevis embryos and the effects on development were monitored during early cleavage by morphological analysis. Scanning electron microscopy revealed the formation of large cells on the injected side of the embryos. Such large cells were not present in controls and thus were considered to have been formed as a consequence of delayed cleavage. Delay of cleavage was obtained with as little as 1 ng of membrane protein giving a ratio of membrane protein to embryo protein of 1:10(5). Cytological observations of microinjected embryos confirmed the occurrence of delayed cytokinesis and suggested that nuclear division became asynchronous. Since rough microsomes from proliferating tissues (i.e., livers with primary tumors and livers undergoing regeneration) showed little or no effect on cytokinesis after microinjection into early embryos, we conclude that cytoplasmic membranes may exhibit cell-cycle-specific properties important for normal development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0829-8211
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1290-300
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Cytoplasmic membrane transplants in early Xenopus embryos indicate cell-cycle-specific effects.
pubmed:affiliation
Département d'anatomie, Faculté de médecine, Université de Montréal, Qué., Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't