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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5639
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pubmed:dateCreated |
2003-9-12
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pubmed:abstractText |
Cooling of blood platelets clusters the von Willebrand factor receptor complex. Macrophage alphaMbeta2 integrins bind to the GPIbalpha subunit of the clustered complex, resulting in rapid clearance of transfused, cooled platelets. This precludes refrigeration of platelets for transfusion, but the current practice of room temperature storage has major drawbacks. We document that alphaMbeta2 is a lectin that recognizes exposed beta-N-acetylglucosamine residues of N-linked glycans on GPIbalpha. Enzymatic galactosylation of chilled platelets blocks alphaMbeta2 recognition, prolonging the circulation of functional cooled platelets. Platelet-associated galactosyltransferase produces efficient galactosylation when uridine diphosphate-galactose is added, affording a potentially simple method for storing platelets in the cold.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylglucosamine,
http://linkedlifedata.com/resource/pubmed/chemical/Galactose,
http://linkedlifedata.com/resource/pubmed/chemical/Galactosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Glycoprotein GPIb-IX...,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Uridine Diphosphate Galactose,
http://linkedlifedata.com/resource/pubmed/chemical/glycoprotein receptor GPIb-IX,
http://linkedlifedata.com/resource/pubmed/chemical/von Willebrand Factor
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
12
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pubmed:volume |
301
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1531-4
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12970565-Acetylglucosamine,
pubmed-meshheading:12970565-Animals,
pubmed-meshheading:12970565-Blood Platelets,
pubmed-meshheading:12970565-Blood Preservation,
pubmed-meshheading:12970565-Carbohydrate Conformation,
pubmed-meshheading:12970565-Cell Line,
pubmed-meshheading:12970565-Cell Survival,
pubmed-meshheading:12970565-Cold Temperature,
pubmed-meshheading:12970565-Female,
pubmed-meshheading:12970565-Galactose,
pubmed-meshheading:12970565-Galactosyltransferases,
pubmed-meshheading:12970565-Glycosylation,
pubmed-meshheading:12970565-Humans,
pubmed-meshheading:12970565-Lectins,
pubmed-meshheading:12970565-Ligands,
pubmed-meshheading:12970565-Macrophage-1 Antigen,
pubmed-meshheading:12970565-Male,
pubmed-meshheading:12970565-Mice,
pubmed-meshheading:12970565-Mice, Inbred C57BL,
pubmed-meshheading:12970565-Monosaccharides,
pubmed-meshheading:12970565-Phagocytosis,
pubmed-meshheading:12970565-Platelet Aggregation,
pubmed-meshheading:12970565-Platelet Glycoprotein GPIb-IX Complex,
pubmed-meshheading:12970565-Platelet Membrane Glycoproteins,
pubmed-meshheading:12970565-Platelet Transfusion,
pubmed-meshheading:12970565-Refrigeration,
pubmed-meshheading:12970565-Uridine Diphosphate Galactose,
pubmed-meshheading:12970565-von Willebrand Factor
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pubmed:year |
2003
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pubmed:articleTitle |
Glycosylation restores survival of chilled blood platelets.
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pubmed:affiliation |
Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. khoffmeister@rics.bwh.harvard.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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