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rdf:type |
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lifeskim:mentions |
umls-concept:C0023821,
umls-concept:C0037083,
umls-concept:C0085201,
umls-concept:C0184512,
umls-concept:C0242738,
umls-concept:C0256371,
umls-concept:C1515877,
umls-concept:C1706853,
umls-concept:C1710082,
umls-concept:C1879547,
umls-concept:C1879748
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pubmed:issue |
48
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pubmed:dateCreated |
2003-11-24
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pubmed:abstractText |
ATP-binding cassette transporter A1 (ABCA1) plays an essential role in the helical apolipoprotein-mediated assembly of high density lipoprotein, and the apolipoporteins stabilize ABCA1 against calpain-mediated degradation during the reaction ((2002) J. Biol. Chem. 277, 22426-22429). Protein kinase C (PKC) inhibitors suppressed both ABCA1 stabilization and cellular lipid release mediated by apolipoprotein A-I (apoA-I) but not ABCA1 increase by calpain inhibitors. The increase of ABCA1 and the cellular lipid release by apoA-I were both suppressed by a phosphatidylcholine phospholipase C (PC-PLC) inhibitor but not by the inhibitors of phosphatidylinositol-PLC and phosphatidylinositol 3-kinase. A protein phosphatase inhibitor further enhanced the ABCA1 increase by apoA-I. Biochemical and microscopic evidence indicated that apoA-I activated PKC alpha, and phosphorylation of ABCA1 was directly demonstrated by apoA-I via PKC. Finally, digestion of sphingomyelin increased ABCA1, and a PC-PLC inhibitor suppressed it. We conclude that apoA-I activates PKC alpha by PC-PLC-mediated generation of diacylglycerol initiated by the removal of cellular sphingomyelin ((2002) J. Biol. Chem. 277, 44709-44714), and subsequently phosphorylates and stabilizes ABCA1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP binding cassette transporter 1,
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoprotein A-I,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Calpain,
http://linkedlifedata.com/resource/pubmed/chemical/Diglycerides,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, HDL,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PRKCA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sphingomyelins,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/phosphatidylcholine-specific...
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
47890-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12952980-ATP-Binding Cassette Transporters,
pubmed-meshheading:12952980-Apolipoprotein A-I,
pubmed-meshheading:12952980-Apolipoproteins,
pubmed-meshheading:12952980-Blotting, Western,
pubmed-meshheading:12952980-Calpain,
pubmed-meshheading:12952980-Cell Line,
pubmed-meshheading:12952980-Diglycerides,
pubmed-meshheading:12952980-Dose-Response Relationship, Drug,
pubmed-meshheading:12952980-Enzyme Activation,
pubmed-meshheading:12952980-Enzyme Inhibitors,
pubmed-meshheading:12952980-Fibroblasts,
pubmed-meshheading:12952980-Green Fluorescent Proteins,
pubmed-meshheading:12952980-Humans,
pubmed-meshheading:12952980-Lipid Metabolism,
pubmed-meshheading:12952980-Lipoproteins, HDL,
pubmed-meshheading:12952980-Luminescent Proteins,
pubmed-meshheading:12952980-Models, Biological,
pubmed-meshheading:12952980-Phosphorylation,
pubmed-meshheading:12952980-Protein Kinase C,
pubmed-meshheading:12952980-Protein Kinase C-alpha,
pubmed-meshheading:12952980-RNA, Messenger,
pubmed-meshheading:12952980-Recombinant Fusion Proteins,
pubmed-meshheading:12952980-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12952980-Signal Transduction,
pubmed-meshheading:12952980-Sphingomyelins,
pubmed-meshheading:12952980-Time Factors,
pubmed-meshheading:12952980-Type C Phospholipases
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pubmed:year |
2003
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pubmed:articleTitle |
Apolipoprotein A-I activates protein kinase C alpha signaling to phosphorylate and stabilize ATP binding cassette transporter A1 for the high density lipoprotein assembly.
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pubmed:affiliation |
Department of Biochemistry, Cell Biology, and Metabolism, Nagoya City University Graduate School of Medical Sciences, Kawasumi 1, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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