Linked Life Data

12949905

Source:http://linkedlifedata.com/resource/pubmed/id/12949905

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-9-1
pubmed:abstractText
We have demonstrated previously that the transcription factor activator protein-1 (AP-1) complex is translocated into mitochondria into the nucleus in murine hippocampus after systemic kainate injection (Ogita et al. [2002] J. Neurosci. 22:2561-2570). The present study investigates whether the mitochondrial AP-1 complex translocated in response to kainate treatment binds to AP-1-like sites located at the non-coding region of the mitochondrial genome in mouse hippocampus. There are 10 sites with sequences similar to the nuclear AP-1 site in the non-coding region. Of 10 pieces (MT-1-MT-10) of synthesized double-stranded oligonucleotides, each containing a mitochondrial AP-1-like site, MT-3, MT-4, and MT-9 were effective in inhibiting mitochondrial AP-1 DNA binding enhanced by kainate. Electrophoresis mobility shift analysis using radiolabeled MT-3 and MT-9 probes demonstrated marked enhancement with binding of these 2 probes in hippocampal mitochondrial extracts prepared 2-6 hr after kainate treatment. Unlabeled AP-1 probe was more potent than unlabeled MT-9 probe in inhibiting the mitochondrial MT-9 binding. Supershift analysis revealed participation of particular Fos/Jun family proteins, such as c-Fos, Fos-B, c-Jun, Jun-B, and Jun-D, in MT-9 binding in hippocampal mitochondrial extracts prepared 4 hr after kainate treatment. Immunoprecipitation analysis using anti-c-Fos antibody demonstrated that c-Fos associated with the mitochondrial genome in hippocampal mitochondria prepared from kainate-treated animals. These results suggest that the AP-1 complex expressed by in vivo kainate treatment would bind to AP-1-like sites in the non-coding region of the mitochondrial genome after translocation into mitochondria from murine hippocampus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0360-4012
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
794-802
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12949905-Animals, pubmed-meshheading:12949905-Antibodies, pubmed-meshheading:12949905-Area Under Curve, pubmed-meshheading:12949905-Binding, Competitive, pubmed-meshheading:12949905-Binding Sites, pubmed-meshheading:12949905-DNA-Binding Proteins, pubmed-meshheading:12949905-Dose-Response Relationship, Drug, pubmed-meshheading:12949905-Electrophoretic Mobility Shift Assay, pubmed-meshheading:12949905-Excitatory Amino Acid Agonists, pubmed-meshheading:12949905-Exoribonucleases, pubmed-meshheading:12949905-Gene Expression Regulation, pubmed-meshheading:12949905-Genome, pubmed-meshheading:12949905-Hippocampus, pubmed-meshheading:12949905-Kainic Acid, pubmed-meshheading:12949905-Male, pubmed-meshheading:12949905-Mice, pubmed-meshheading:12949905-Mice, Inbred Strains, pubmed-meshheading:12949905-Mitochondria, pubmed-meshheading:12949905-Polymerase Chain Reaction, pubmed-meshheading:12949905-Precipitin Tests, pubmed-meshheading:12949905-Promoter Regions, Genetic, pubmed-meshheading:12949905-Sequence Homology, pubmed-meshheading:12949905-Time Factors, pubmed-meshheading:12949905-Transcription Factor AP-1
pubmed:year
2003
pubmed:articleTitle
Transcription factor activator protein-1 expressed by kainate treatment can bind to the non-coding region of mitochondrial genome in murine hippocampus.
pubmed:affiliation
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan. ogita@pharm.setsunan.ac.jp
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't