Linked Life Data

12942648

Source:http://linkedlifedata.com/resource/pubmed/id/12942648

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-8-28
pubmed:abstractText
A mutation resulting in substitution of positively charged Lys53 with negatively charged Glu in the coat protein was introduced in the infectious cDNA copy of the genome of wild-type tobacco mosaic virus strain U1. Kinetic analysis of long-distance virus transport in plants showed that systemic distribution of the mutant virus was delayed by 5-6 days as compared with the wild-type one. On evidence of RNA sequencing in the mutant progeny, Glu50 of the coat protein was substituted with Lys after passage I to compensate for the loss of the positive charge at position 53. Electron microscopy revealed atypical inclusions (rodlike structures, multiple electron-dense globular particles) in the nuclear interchromatin space of leaf mesophyll cells infected with the mutant but not with the wild-type virus.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0026-8984
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
742-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
[A point mutation in the coat protein gene affects long distance transport of the tobacco mosaic virus].
pubmed:affiliation
Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Moscow, 127550 Russia.
pubmed:publicationType
Journal Article, English Abstract