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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2003-8-27
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pubmed:abstractText |
To investigate whether specific-pathogen-free (SPF) shrimps are asymptomatic carriers of white spot syndrome virus (WSSV), we used a WSSV-specific DNA microarray to measure WSSV gene expression in SPF and WSSV-infected shrimps. Three WSSV genes were found to be relatively highly expressed in SPF shrimps. Reverse transcription-PCR using nested primers as well as real-time detection confirmed that these genes have no detectable counterparts in GenBank; structural analysis of the putative proteins revealed helix-loop-helix and leucine zipper motifs. Viral sequences could be PCR amplified from genomic DNA of SPF shrimp, further supporting the suggestion that these shrimps are asymptomatic carriers.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-10782350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-10907134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-10986648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-11448154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-11533207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-11689662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-12093036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-12113310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-1689846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-1846042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-8503177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-8800675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12941929-9225059
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10162-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12941929-Animals,
pubmed-meshheading:12941929-DNA Viruses,
pubmed-meshheading:12941929-Decapoda (Crustacea),
pubmed-meshheading:12941929-Genes, Viral,
pubmed-meshheading:12941929-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:12941929-Open Reading Frames,
pubmed-meshheading:12941929-RNA, Viral,
pubmed-meshheading:12941929-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12941929-Virus Latency
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pubmed:year |
2003
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pubmed:articleTitle |
Identification of white spot syndrome virus latency-related genes in specific-pathogen-free shrimps by use of a microarray.
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pubmed:affiliation |
Animal Health Biotechnology, Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore 117604.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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