Source:http://linkedlifedata.com/resource/pubmed/id/12941839
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
16
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pubmed:dateCreated |
2003-8-27
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pubmed:abstractText |
Interleukin-10 (IL-10) is an immunosuppressive cytokine produced by T lymphocytes and drawing attention as an inhibitor of tumor angiogenesis. In this study, we investigated antiangiogenic and tumor suppressive effects of IL-10 in ovarian cancer cells. mIL-10-expressing plasmid was transferred into two ovarian cancer cell lines, SHIN-3 [vascular endothelial growth factor (VEGF) producing] and KOC-2S (non-VEGF producing). After selection, mIL-10-expressing cells were obtained as SHIN-3/mIL-10 and KOC-2S/mIL-10. No significant differences were observed in in vitro growth properties between mIL-10-expressing cells and control (luciferase expressing) cells in either KOC-2S or SHIN-3. The angiogenic activities of mIL-10-expressing cells were measured by dorsal air sac assay, which detected the number of newly formed blood vessels within a chamber in vivo. In addition, tumor formation was evaluated by s.c. tumor transplantation, and survival was monitored after i.p. injection of ovarian cancer cells into BALB/c nude mice. Both in vivo angiogenic activity and tumor growth were significantly inhibited in SHIN-3/mIL-10 cells compared with the control. Moreover, peritoneal dissemination was inhibited, and the survival period was significantly prolonged (mean survival days > 90 versus 36). In contrast, in the case of KOC-2S cells, no significant differences were observed in any of the parameters tested. These results indicate that IL-10 has suppressive effects on angiogenesis, tumor growth, and peritoneal dissemination of VEGF-producing ovarian cancer cells. Although the mechanisms of the antiangiogenic effect of IL-10 are still unclear, the potential usefulness of IL-10-mediated gene therapy of ovarian cancer was suggested.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0008-5472
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pubmed:author |
pubmed-author:HanazonoYutakaY,
pubmed-author:KohnoTakahiroT,
pubmed-author:KumeAkihiroA,
pubmed-author:MatsushitaTakashiT,
pubmed-author:MizukamiHiroakiH,
pubmed-author:OkadaTakashiT,
pubmed-author:OzawaKeiyaK,
pubmed-author:SagaYasushiY,
pubmed-author:SatoIkuoI,
pubmed-author:ShimpoMasahisaM,
pubmed-author:SuzukiMitsuakiM,
pubmed-author:TakeiYujiY
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5091-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12941839-Animals,
pubmed-meshheading:12941839-Endothelial Growth Factors,
pubmed-meshheading:12941839-Female,
pubmed-meshheading:12941839-Gene Therapy,
pubmed-meshheading:12941839-Humans,
pubmed-meshheading:12941839-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:12941839-Interleukin-10,
pubmed-meshheading:12941839-Lymphokines,
pubmed-meshheading:12941839-Mice,
pubmed-meshheading:12941839-Mice, Inbred BALB C,
pubmed-meshheading:12941839-Neovascularization, Pathologic,
pubmed-meshheading:12941839-Ovarian Neoplasms,
pubmed-meshheading:12941839-Tumor Cells, Cultured,
pubmed-meshheading:12941839-Vascular Endothelial Growth Factor A,
pubmed-meshheading:12941839-Vascular Endothelial Growth Factors
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pubmed:year |
2003
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pubmed:articleTitle |
Interleukin-10-mediated inhibition of angiogenesis and tumor growth in mice bearing VEGF-producing ovarian cancer.
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pubmed:affiliation |
Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, 329-0498 Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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