| pubmed-article:12902268 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12902268 | lifeskim:mentions | umls-concept:C0006304 | lld:lifeskim |
| pubmed-article:12902268 | lifeskim:mentions | umls-concept:C0020202 | lld:lifeskim |
| pubmed-article:12902268 | lifeskim:mentions | umls-concept:C0070828 | lld:lifeskim |
| pubmed-article:12902268 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:12902268 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:12902268 | lifeskim:mentions | umls-concept:C1579762 | lld:lifeskim |
| pubmed-article:12902268 | lifeskim:mentions | umls-concept:C0009491 | lld:lifeskim |
| pubmed-article:12902268 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:12902268 | lifeskim:mentions | umls-concept:C0182400 | lld:lifeskim |
| pubmed-article:12902268 | lifeskim:mentions | umls-concept:C0386577 | lld:lifeskim |
| pubmed-article:12902268 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:12902268 | pubmed:dateCreated | 2003-8-6 | lld:pubmed |
| pubmed-article:12902268 | pubmed:abstractText | Real-time PCR provides a means of detecting and quantifying DNA targets by monitoring PCR product accumulation during cycling as indicated by increased fluorescence. A number of different approaches can be used to generate the fluorescence signal. Three approaches-SYBR Green I (a double-stranded DNA intercalating dye), 5'-exonuclease (enzymatically released fluors), and hybridization probes (fluorescence resonance energy transfer)-were evaluated for use in a real-time PCR assay to detect Brucella abortus. The three assays utilized the same amplification primers to produce an identical amplicon. This amplicon spans a region of the B. abortus genome that includes portions of the alkB gene and the IS711 insertion element. All three assays were of comparable sensitivity, providing a linear assay over 7 orders of magnitude (from 7.5 ng down to 7.5 fg). However, the greatest specificity was achieved with the hybridization probe assay. | lld:pubmed |
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| pubmed-article:12902268 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:12902268 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12902268 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12902268 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:12902268 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:12902268 | pubmed:author | pubmed-author:RobertsF JFJ | lld:pubmed |
| pubmed-article:12902268 | pubmed:author | pubmed-author:HadfieldT LTL | lld:pubmed |
| pubmed-article:12902268 | pubmed:author | pubmed-author:NewbyD TDT | lld:pubmed |
| pubmed-article:12902268 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12902268 | pubmed:volume | 69 | lld:pubmed |
| pubmed-article:12902268 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12902268 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12902268 | pubmed:pagination | 4753-9 | lld:pubmed |
| pubmed-article:12902268 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:12902268 | pubmed:meshHeading | pubmed-meshheading:12902268... | lld:pubmed |
| pubmed-article:12902268 | pubmed:meshHeading | pubmed-meshheading:12902268... | lld:pubmed |
| pubmed-article:12902268 | pubmed:meshHeading | pubmed-meshheading:12902268... | lld:pubmed |
| pubmed-article:12902268 | pubmed:meshHeading | pubmed-meshheading:12902268... | lld:pubmed |
| pubmed-article:12902268 | pubmed:meshHeading | pubmed-meshheading:12902268... | lld:pubmed |
| pubmed-article:12902268 | pubmed:meshHeading | pubmed-meshheading:12902268... | lld:pubmed |
| pubmed-article:12902268 | pubmed:meshHeading | pubmed-meshheading:12902268... | lld:pubmed |
| pubmed-article:12902268 | pubmed:meshHeading | pubmed-meshheading:12902268... | lld:pubmed |
| pubmed-article:12902268 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12902268 | pubmed:articleTitle | Real-time PCR detection of Brucella abortus: a comparative study of SYBR green I, 5'-exonuclease, and hybridization probe assays. | lld:pubmed |
| pubmed-article:12902268 | pubmed:affiliation | Biotechnology Department, Idaho National Engineering and Environmental Laboratory, Idaho Falls, Idaho 83415. Armed Forces Institute of Pathology, Washington, D.C. 20306, USA. newbdt@inel.gov | lld:pubmed |
| pubmed-article:12902268 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12902268 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:12902268 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:12902268 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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