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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2003-8-6
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pubmed:abstractText |
Real-time PCR provides a means of detecting and quantifying DNA targets by monitoring PCR product accumulation during cycling as indicated by increased fluorescence. A number of different approaches can be used to generate the fluorescence signal. Three approaches-SYBR Green I (a double-stranded DNA intercalating dye), 5'-exonuclease (enzymatically released fluors), and hybridization probes (fluorescence resonance energy transfer)-were evaluated for use in a real-time PCR assay to detect Brucella abortus. The three assays utilized the same amplification primers to produce an identical amplicon. This amplicon spans a region of the B. abortus genome that includes portions of the alkB gene and the IS711 insertion element. All three assays were of comparable sensitivity, providing a linear assay over 7 orders of magnitude (from 7.5 ng down to 7.5 fg). However, the greatest specificity was achieved with the hybridization probe assay.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-10631501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-10735245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-10865155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-10878051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-10921983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-11128080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-11162079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-11525995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-11756688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-1377903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-1495123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-2113907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-2254243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-2272943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-6779513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-7650203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-7852552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-8586678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-8633866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-8760325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-8810054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-8994660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-9204307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12902268-9734029
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4753-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2003
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pubmed:articleTitle |
Real-time PCR detection of Brucella abortus: a comparative study of SYBR green I, 5'-exonuclease, and hybridization probe assays.
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pubmed:affiliation |
Biotechnology Department, Idaho National Engineering and Environmental Laboratory, Idaho Falls, Idaho 83415. Armed Forces Institute of Pathology, Washington, D.C. 20306, USA. newbdt@inel.gov
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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