Source:http://linkedlifedata.com/resource/pubmed/id/12895574
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
18
|
| pubmed:dateCreated |
2003-8-4
|
| pubmed:abstractText |
A protein micro-array, called a "cell chip" was constructed by using a photo-reactive polymer for a cell-adhesion assay. Various amounts of albumin or fibronectin were covalently immobilized on a polystyrene dish using a micro-spotter with a dip pen. First, poly(acrylic acid) carrying azidophenyl groups was synthesized as the photo-reactive polymer. Secondly, the aqueous solution of a photo-reactive polymer (several nanoliters) was cast using the dip pen of the micro-spotter and dried in air. Subsequently, aqueous solutions of proteins were cast on the same place using the micro-spotter. After drying, the dish was irradiated with ultraviolet light. Finally, the immobilization was confirmed by staining with a dye. The immobilization was stable even after washing with Tween-20. The protein-immobilized area depended on the manipulation of the micro-spotter and the size of the dip pen. Subsequently, cell adhesion on the photo-immobilized protein micro-array was investigated. The adhesion behavior of cells depended on the kind of immobilized proteins and the kind of cells. The protein micro-array will be useful for cell diagnosis and for the selection of biomaterials to regulate cell behavior.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acrylic Resins,
http://linkedlifedata.com/resource/pubmed/chemical/Coated Materials, Biocompatible,
http://linkedlifedata.com/resource/pubmed/chemical/Fibronectins,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin, Bovine,
http://linkedlifedata.com/resource/pubmed/chemical/carbopol 940
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0142-9612
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
24
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3021-6
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12895574-Acrylic Resins,
pubmed-meshheading:12895574-Animals,
pubmed-meshheading:12895574-COS Cells,
pubmed-meshheading:12895574-Cell Adhesion,
pubmed-meshheading:12895574-Cell Culture Techniques,
pubmed-meshheading:12895574-Cell Line,
pubmed-meshheading:12895574-Cercopithecus aethiops,
pubmed-meshheading:12895574-Coated Materials, Biocompatible,
pubmed-meshheading:12895574-Equipment Design,
pubmed-meshheading:12895574-Equipment Failure Analysis,
pubmed-meshheading:12895574-Fibronectins,
pubmed-meshheading:12895574-Macrophages,
pubmed-meshheading:12895574-PC12 Cells,
pubmed-meshheading:12895574-Photochemistry,
pubmed-meshheading:12895574-Polymers,
pubmed-meshheading:12895574-Protein Array Analysis,
pubmed-meshheading:12895574-Rats,
pubmed-meshheading:12895574-Serum Albumin, Bovine
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Preparation of a protein micro-array using a photo-reactive polymer for a cell-adhesion assay.
|
| pubmed:affiliation |
Kanagawa Academy of Science and Technology, KSP East 309, Sakado 3-2-1, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|